Structural and Functional Evaluation of Blood-Ocular-Barrier

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591953
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Aichi Medical University
• Principal Investigator: ZAKO Masahiro 愛知医科大学, 医学部, 教授 (80298596)
• Co-Investigator(Kenkyū-buntansha): YONEDA Masahiko 愛知県立看護大学, 看護学部, 教授 (80201086)
• Project Period (FY): 2010 – 2012
• Keywords: 血液房水関門 / ぶどう膜炎 / 腫瘍壊死因子 / 抗腫瘍壊死因子抗体 / マトリックスメタ ロプロテアーゼ(MMP)

Research Abstract

Induction of matrix metalloproteinases (MMPs) in non-pigmented ciliary epithelium (HNPCECs) by tumor necrosis factor-alpha (TNF-α) was counteracted by infliximab. Immunostaining showed that the MMPs degraded claudin-1 and occludin, major components of the tight junctions in the blood-aqueous barrier, in HNPCECs and in non-pigmented ciliary epithelial cells of the swine ciliary body.

Research Products

• [Journal Article] Infliximab counteracts tumor necrosis factor-α-enhanced induction of matrix metalloproteinases that degrade claudin and occludin in non-pigmented ciliary epithelium.
  • Author(s): Yamada H, Yoneda M, Inaguma S, Watanabe D, Banno S, Yoshikawa K, Mizutani K, Iwaki M, Zako M.
  • Journal Title: Biochem Pharmacol. Volume: (in press)
  • DOI: doi:pii: S0006-2952(13)00230-X.
• [Presentation] Infliximab attenuates tumornecrosis factor-α-induced alterations in non-pigmented ciliary epithelium. (2013)
  • Author(s): Yamada H, Yoneda M, Inaguma S, Watanabe D, Banno S, Yoshikawa K, Mizutani K, Iwaki M, Zako M.
  • Organizer: ARVO
  • Place of Presentation: Seattle
  • Year and Date: 2013-05-05