2012 Fiscal Year Final Research Report
Differentiation of retinal progenitors derived from ES cells and iPS cells.
| Project/Area Number |
22591969
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
SUZUKI Noboru 聖マリアンナ医科大学, 医学部, 教授 (40235982)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 眼発生 / 再生医学 / iPS 細胞 |
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| Research Abstract |
The main cells that make up the retina, are photoreceptor cells, retinal ganglion cells, and bipolar cells. The photoreceptor cells are classified into rod-photoreceptor cells for sensing light and dark, and the cone photoreceptors responsible for color vision.In the course of embryonal development and somatic regeneration occur, differentiation from retinal progenitor cells into bipolar cells, retinal ganglion cells, cone photoreceptors and rod-photoreceptor cells has been extensively studied. Nevertheless, molecules involved in the differentiation processes have not been fully clarified.We have introduced a gene pax6 which is one of the most important transcription factors involved in the eye development into mouse iPS cells and mouse ES cells. After limitingdilution culture of the pax6 transfected cells, we have established several retinal neural progenitor cell lines expressing simultaneously nestin, musashi1, six3, and chx10, a transcription factor which is importantly invod in differentiation of retinal progenitor cells. The retinal progenitor cells we have established from iPS cells are 50% positive for cell surface CD73, a retinal progenitor cell marker, and express a moderate amount of rhodopsin protein upon unstimulated condition. The cells differentiate into cells expressing green-opsin, blue-opsin, and red-opsin in response to various chemokines and growth factors.In contrast, cell lines established from ES cells with the same procedure differentiate into retinal ganglion cells predominantly. Collectively, we found that differentiation of photoreceptor precursor cells into cone photoreceptors and rod-photoreceptor cells areregulated by respective chemokines and growth factors.
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Research Products
(41 results)
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[Journal Article] Serum level of soluble triggering receptor expressed on myeloid cells-1 as a biomarker of disease activity in relapsing polychondritis.2013
Author(s)
Sato T, Yamano Y, Tomaru U, Shimizu Y, Ando H, Okazaki T, Nagafuchi H, ShimizuJ, Ozaki S, Miyazawa T, Yudoh K, Oka H, Suzuki N.
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Journal Title
Peer Reviewed
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[Journal Article] Skewed TGFβ/Smad signaling pathway of T cells in patients with Behcet's disease.2013
Author(s)
Shimizu J, Izumi T, Arimitsu N, FujiwaraN, Ueda Y, Wakisaka S, Yoshikawa H, Kaneko F, Suzuki T, Takai K, Suzuki N.
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Journal Title
Peer Reviewed
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[Journal Article] The Relapsing Polychondritis DiseaseActivity Index: development of a disease activity score for relapsing polychondritis.2013
Author(s)
Arnaud L, Devilliers H, Peng SL, MathianA, Costedoat-chalumeau N, Buckner J, Dagna L, Michet C, Sharma A, Cervera R,Haroche J, Papo T, D'Cruz D, Arlet P, Zwerina J, Belot A, Suzuki N, Harle JR, Moots R, Jayne D, Hachulla E, Marie I, Tanaka T, Lebovics R, Scott D, Kucharz EJ, Birchall M, Kong KO, Gorochov G, Amoura Z.
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Journal Title
Peer Reviewed
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[Journal Article] Overexpression of SPACIA1/ SAAL1, a new gene that is involved in synoviocyte proliferation2011
Author(s)
Sato T, Fujii R, Konomi K, Yagishita N, Aratani S, Araya N, Aono H, Yudoh K, Suzuki N, Moroe B, Yamano Y, Nishioka K,Nakajima T.
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Journal Title
accelerates the progression of synovitis in mice and humans. Arthritis & Rheumatism
Volume: 63(12)
Pages: 3833-3842
Peer Reviewed
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[Journal Article] E3 ubiquitin ligasesynoviolin is involved in liver fibrogenesis.2010
Author(s)
Hasegawa D, Fujii R, Yagishita N, Matsumoto N, Aratani S, Izumi T, Azakami K, Nakazawa M, Fujita H, Sato T, Araya N, Koike J, Tadokoro M, Suzuki N, Nagata K, Senoo H, Freidman LS, Nishioka K, Yamano Y, Itoh F, Nakajima T.
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Journal Title
PloS ONE
Volume: 5(10)
Pages: e13590
Peer Reviewed
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