2012 Fiscal Year Final Research Report
The establishment of the effective culture method of retinal stem / progenitor cells derived from iris tissue and the physiologic function test of differentiated cells
| Project/Area Number |
22591970
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Fujita Health University |
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Principal Investigator |
YAMAMOTO Naoki 藤田保健衛生大学, 共同利用研究施設, 准教授 (00267957)
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| Co-Investigator(Kenkyū-buntansha) |
TANIKAWA Atsuhiro 藤田保健衛生大学, 医学部, 准教授 (40324412)
OHKUMA Mahito 藤田保健衛生大学, 医学部, 講師 (50329710)
HORIGUCHI Masayuki 藤田保健衛生大学, 医学部, 教授 (70209295)
MIYACHI Ei-ichi 藤田保健衛生大学, 医学部, 教授 (90129685)
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| Co-Investigator(Renkei-kenkyūsha) |
SENDA Takao 藤田保健衛生大学, 医学部, 教授 (10187875)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 眼発生 / 再生医学(組織幹細胞による網膜再生) |
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| Research Abstract |
When we coated the culture plate surface with lamina collagen gel, the culture cells which were derived from mouse iris tissue were expressed the tissue stem cell marker. Then, we were cultured the nerve-like cells effectively by sorting cells derived from culture human iris tissue. When we cultured tissue stem / progenitor cells derived from human iris tissue in a retinoic acid containing nutrient medium, the cells were differentiated the expressed marker protein of the neural retina cell. Furthermore, the physiologic function of cells were evaluated by the patch clamp method, the cells weredetected with a sodium channel and the potassium channel.
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