2012 Fiscal Year Final Research Report
Establishment of a novel method for retinal cell differentiation using human cells derived from child eyeballs
| Project/Area Number |
22591975
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
NISHINA Sachiko 独立行政法人国立成育医療研究センター, 感覚器・形態外科部 眼科, 医師 (40237954)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 視覚障害 / 先天性疾患 / 再生医療 / ES細胞 / 網膜芽細胞種 / 低分子量化合物 / p38MAP / キナーゼ / SB203580 |
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| Research Abstract |
We aimed to identify small molecules that can induce neuronal cell differentiation. As the results, we show that 1) the p38 MAPK-specific inhibitor SB203580 blocks the spontaneous differentiation of ES cells into cardiomyocytes, and instead induces the differentiation of these ES cells into neurons 2) p38 regulates BMP2 gene expression through the transcription factor MEF2C, and 3) JNK signaling pathway regulates axon elongation. Furthermore, 4) we screened 1,600 well-characterized small molecule compounds and identified about 20 drugs that stimulate neuronal differentiation.
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[Journal Article] Ophthalmic features of CHARGE syndrome with CHD7 mutations.2012
Author(s)
Nishina S, Kosaki R, Yagihashi T, Azuma N, Okamoto N, Hatsukawa Y, Kurosawa K, Yamane T, Mizuno S, Tsuzuki K, Kosaki K
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Journal Title
Am J Med Genet A
Volume: 158
Pages: 514-518
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[Journal Article] Two novel mutations in the EYS Gene are possible major causes of autosomal recessive retinitis pigmentosa in the Japanese population.2012
Author(s)
Hosono K, Ishigami C, Takahashi M, Park DH, Hirami Y, Nakanishi H, Ueno S, Yokoi T, Hikoya A, Fujita T, Zhao Y, Nishina S, Shin JP, Kim IT, Yamamoto S, Azuma N, Terasaki H, Sato M, Kondo M, Minoshim S, Hotta Y
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Journal Title
PLoS ONE
Volume: 7(2)
Pages: e31036
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