2012 Fiscal Year Final Research Report
Functional roles of sphingosine-1-phosphate in mesenchymal stem cell migration and proliferation and bone metabolism
| Project/Area Number |
22592000
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Saitama Medical University |
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Principal Investigator |
SATO Tomoya 埼玉医科大学, 医学部, 助教 (10445132)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 間葉系幹細胞 / 骨代謝 / 骨芽細胞 / 再生医学 |
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| Research Abstract |
During bone formation, osteoblasts are derived from mesenchymal stem cells (MSCs) present in the bone marrow. Recent studies have indicated that sphingosine-1-phosphate (S1P) plays a vital role in various tissues; however, its role in bone metabolism remains unclear. Here, we demonstrated that S1P induces proliferation and migration of MSCs. We detected the mRNA expression of S1P receptor 1 (S1PR1) via reverse transcription polymerase chain reaction (RT-PCR) and determined whether S1P induces proliferation of MSCs. Treatment of MSCs with S1P for 24 h stimulated a dose-dependent increase in DNA synthesis. These data demonstrated that S1P is mitogenic to MSCs in vitro. S1P produced by osteoclast precursors during differentiation may function in a paracrine manner to MSCs. To explore this possibility, we examined the chemotactic effects of S1P on MSCs. S1P increased chemotaxis of MSCs; however, the differentiation of MSC to osteoblasts was not affected by S1P. These results suggest that S1P plays a possible role in regulating the proliferation and migration of MSCs.
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