Maintenance mechanism of oral homeostasis revealed by fine scale analysis of microbiota

2012 Fiscal Year Final Research Report

• Project/Area Number: 22592032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MARUYAMA Fumito 東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (30423122)
• Co-Investigator(Kenkyū-buntansha): SAKURAI Atsuo 東京歯科大学, 小児歯科講座, 助教 (90431759) ; NAKAGAWA Ichiro 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (70294113) ; KUROKAWA Ken 東京工業大学, 大学院・生命理工学研究科, 教授 (20343246)
• Project Period (FY): 2010 – 2012
• Keywords: 細菌叢 / 新型シーケンサー / 恒常性

Research Abstract

The oral cavity is exposed to many complex substances and is the first door for bacteria to invade the body. The goal of this study is to perform the oral flora analysis precisely and is intended to clarify the mechanism that how constancy of the oral microbial environment is maintained. The oral environment changes complicatedly, and there is no data about Archaea. Therefore comparative bacterial community analysis was carried out using normal oral flora, caries and periodontal disease, aimed at the elucidation of the oral constancy maintenance mechanism through isolation and genome determination of the key bacteria to the homeostasis. As a result, in the children of around 10 years old, the bacteria were not different with those of an adult. On the other hand, as a result of the genome analysis of the isolated strains, bacterial survival in the niche could be affected greatly by CRISPR which was a bacterial acquired immunity mechanism. The knowledge becomes the base of future metagenome analysis.

Research Products

• [Journal Article] Diversity and Universality of Capsular Polysaccharide Synthesis Gene Clusters in Streptococcus suis (2013)
  • Author(s): D. Takamatsu and F. Maruyama
  • Journal Title: Appl. Environ. Microbiol Volume: 79 Pages: 2493
• [Journal Article] GeneticAnalysis of Capsular Polysaccharide Synthesis Gene Clusters from All Serotypes of Streptococcus suis:Potential Mechanisms for theGeneration of Capsular Variation (2013)
  • Author(s): M. Okura, D. Takamatsu, F.Maruyama, T. Nozawa, I. Nakagawa, M. Osaki, T. Sekizaki, M. Gottschalk,Y. Kumagai, and S. Hamada
  • Journal Title: Appl. Environ. Microbiol Volume: 79 Pages: 2796-2806
  • Peer Reviewed
• [Journal Article] Complete genome sequence of Propionibacterium acnes isolate from sarcoidosis patient (2013)
  • Author(s): K. Minegishi, C. Aikawa, A.Furukawa, T. Watanabe, T. Nakano, Y. Ogura, Y. Ohtsubo, K. Kurokawa, T. Hayashi, F. Maruyama, I. Nakagawa,Y. Eishi
  • Journal Title: Genome Announc Volume: 1 Pages: e00016-12
  • Peer Reviewed
• [Journal Article] Complete Genome Sequence of Acidovorax sp.KKS102, a Polychlorinated Biphenyl-Degrading strain (2012)
  • Author(s): Y. Ohtsubo, F. Maruyama, H. Mitsui, Y. Nagata, M. Tsuda
  • Journal Title: J. Bacteriol Volume: 194 Pages: 6970-1
  • Peer Reviewed
• [Journal Article] Phytate addition to soil induces changes in the abundance and expression of Bacillus β-propeller phytase genes in the rhizosphere (2012)
  • Author(s): M. Jorquera, N. Saavedra, F.Maruyama, A. Richardson, D. Crowley, R. Catrilaf, E. Henriquez, M. Mora
  • Journal Title: FEMS Microbiol. Ecol Volume: 83 Pages: 352-60
  • Peer Reviewed
• [Journal Article] The small GTPases Rab9A and Rab23 function at distinct steps in autophagyduring Group A Streptococcus infection. (2012)
  • Author(s): -T. Nozawa, C. Aikawa, A. Goda, F.Maruyama, S. Hamada, I. Nakagawa
  • Journal Title: Cell. Microbiol Volume: 14 Pages: 1149-1165
  • Peer Reviewed
• [Journal Article] Complete Genome Sequence of the serotype k Streptococcus mutans LJ23 (2012)
  • Author(s): C. Aikawa, N. Furukawa, T. Watanabe, K. Minegishi, A. Furukawa, Y. Eishi,K. Oshima, K. Kurokawa, M. Hattori,K. Nakano, *F. Maruyama, I. Nakagawa and T. Ooshima
  • Journal Title: J. Bacteriol Volume: 194 Pages: 2754
  • Peer Reviewed
• [Journal Article] Novel conjugative transferable multiple drug resistance plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from marine aquaculture environment (2012)
  • Author(s): L. Nonaka, F. Maruyama, M.Miyamoto, M. Miyakoshi, K. Kurokawa, M. Masuda
  • Journal Title: Microb.Environ Volume: 27 Pages: 263-272
  • Peer Reviewed
• [Journal Article] Transition metal ions induce carnosinase activity in PepD-homologous protein from Porphyromonas gingivalis TDC60 with type II fimA (2012)
  • Author(s): A. Aoki, Y. Shibata, S. Okano, F.Maruyama, A. Amano, I. Nakagawa,*Y. Abiko
  • Journal Title: Microb. Pathog Volume: 52 Pages: 17-24
  • Peer Reviewed
• [Journal Article] Genomic structure of the cyanobacterium Synechocystis sp. PCC 6803 strain GT-S (2011)
  • Author(s): N. Tajima, S. Sato, F. Maruyama, N.Kaneko, V. Sasaki, K. Kurokawa, H.Ohta, Y. Kanesaki, H. Yoshikawa, S. Tabata, M. Ikeuchi and *N. Sato
  • Journal Title: DNA Res Volume: 18 Pages: 393-399
  • Peer Reviewed
• [Journal Article] Complete Genome Sequence of the Periodontogenic Bacterium Porphyromonas gingivalis TDC60 (2011)
  • Author(s): F. Maruyama, T. Watanabe, T. Nozawa, A. Aoki, S. Okano, Y. Shibata, K. Oshima, K. Kurokawa, M. Hattori, I. Nakagawa, Y. Abiko
  • Journal Title: J. Bacteriol Volume: 193 Pages: 4259-4260
  • Peer Reviewed
• [Journal Article] CRISPR inhibition of prophage acquisition in Streptococcus pyogenes (2011)
  • Author(s): T. Nozawa, N. Furukawa, C. Aikawa, T. Watanabe, B. Haobam, K. Kurokawa, *F. Maruyama, I. Nakagawa
  • Journal Title: PLoS One Volume: 6 Pages: e19543
  • Peer Reviewed
• [Journal Article] The dawning era of comprehensive transcriptome analysis in cellular microbiology (2010)
  • Author(s): C. Aikawa, F. Maruyama, I. Nakagawa
  • Journal Title: Front. Microbiol Volume: 1 Pages: 118
  • Peer Reviewed
• [Journal Article] visualization tool for taxonomic compositions of microbial communities based on 16S rRNA gene sequences (2010)
  • Author(s): H. Mori, F. Maruyama, K. Kurokawa.VITCOMIC
  • Journal Title: BMC Biofinfo Volume: 11 Pages: 332
  • Peer Reviewed
• [Journal Article] Commensal Proteobacteria in colonic CD11b+ cellsinduce intestinal T cell responses (2010)
  • Author(s): -I. Takahashi, F. Maruyama, Y. Kurashima, Y. Goto, T. Obata, I. Nakagawa, *H. Kiyono
  • Journal Title: Int. Immunol Volume: 22
• [Journal Article] The specific behavior of intracellular Streptococcus pyogenes undergone the autophagic degradation is associated withbacterial streptolysin O and host small G proteins Rab5 and Rab7 (2010)
  • Author(s): A. Sakurai, F. Maruyama, J. Funao, T.Nozawa, C. Aikawa, N. Okahashi, S. Shintani, S. Hamada, *T. Ooshima, I.Nakagawa
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 22666-22675
  • Peer Reviewed
• [Book] 化学と生物 (2013)
  • Author(s): 渡辺孝康、中川一路、丸山史人
  • Publisher: 原核生物の新規な獲得免疫機構CRISPR/Cas システム
• [Book] 第2章メタゲノムデータベース. メタゲノム解析技術の最前線. Metagenomics:a new frontier of bacterial community researches (2010)
  • Author(s): 森宙史、丸山史人、*黒川顕
  • Total Pages: 42-53
  • Publisher: CMC 出版,東京
• [Book] 第8章メタゲノムインフォマティクス.難培養微生物研究の最新技術 II - ゲノ ム解析を中心とした最前線と将来展望- Current Technology and Perspectives for Yet-uncultivated Microbial Resources (2010)
  • Author(s): 森宙史、丸山史人、黒川顕
  • Total Pages: 82-91
  • Publisher: CMC 出版,東京
• [Remarks] 東京医科歯科大学・大学院医歯学総合研究科・細菌感染制御学分野
  • URL: http://www.tmd.ac.jp/grad/bac/index.html
• [Remarks] 東京医科歯科大学・大学院医歯学総合研究科・環境遺伝生態学分野
  • URL: https://www.facebook.com/pages/Microbial-Genomics-and-Ecology/135689926563239