2012 Fiscal Year Final Research Report
Transcriptional and post-transcriptional regulation of noradrenaline transporter gene expression by nicotine
| Project/Area Number |
22592066
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
OHYAMA Kazumi 岡山大学, 大学院・医歯薬学総合研究科, 助手 (00253021)
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| Co-Investigator(Kenkyū-buntansha) |
KITAYAMA Shigeo 岡山大学, 大学院・医歯薬学総合研究科, 教授 (80177873)
SOGAWA Norio 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (30236153)
SOGAWA Chiharu 岡山大学, 大学院・医歯薬学総合研究科, 助教 (10253022)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 歯科薬理学 / ニコチン |
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| Research Abstract |
We investigated the effects of nicotine on noradrenaline transporter(NET) expression in PC12 cells derived from rat pheochromocytoma, human neuroblastoma SK-N-SH cells, and rat tissues. Nicotine had no influence on the transcriptional activity of human NET gene constructs transiently transfected in SK-N-SH and PC12 cells. However, nicotine decreased NET mRNA levels within several hours in SK-N-SH cells. In contrast, nicotine up-regulated the mRNA and protein of NET in PC12 cells. A single administration of nicotine increased NET mRNA levels in the adrenal medulla and brain stem of the rat, whereas chronic nicotine treatment for 2 weeks decreased NET mRNA levels. These results suggest that nicotine up- and down-regulates the expression of NET through a posttranscriptional mechanism. The decrease in NET expression induced by nicotine may be ofparticular clinical relevance to the pharmacological action of nicotine.
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