2012 Fiscal Year Final Research Report
Analysis of spatiotemporal pattern in IP^ production in submandibulargland using fluorescent-biosenseor
| Project/Area Number |
22592073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
TOJYO Yosuke 北海道医療大学, 歯学部, 教授 (90111731)
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| Co-Investigator(Kenkyū-buntansha) |
TANIMURA Akihiko 北海道医療大学, 歯学部, 教授 (70217149)
NEZU Akihiro 北海道医療大学, 歯学部, 講師 (00305913)
MORITA Takao 北海道医療大学, 歯学部, 講師 (20326549)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 唾液腺 / IP^ / Ca^ / LIBRA / イメージング |
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| Research Abstract |
We constructed an adenovirus vector that expressed the cytosolic type IP^ biosensor cLIBRAvIIs. The adenovirus vector was transferred to rat submandibular glands by retrograde ductal injection, and we detected the expression of cLIBRAvIIs in submandibular glands. We examined the effects of carbachol (CCh) on the fluorescence ratio of cLIBRAvIIs in dispersed submandibular acinar cells. Stimulation with CCh significantly increased the ratio. Furthermore, we successfully performed in vivo Ca^ imaging in rat submandibular glands expressing the Ca^ biosensor YC-Nano-50.
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