2012 Fiscal Year Final Research Report
Analyses of molecular mechanisms of salivary secretion in salivary acinar cells using gene expression in vivo
| Project/Area Number |
22592075
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
MORITA Takao 北海道医療大学, 歯学部, 講師 (20326549)
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| Co-Investigator(Kenkyū-buntansha) |
NEZU Akihiro 北海道医療大学, 歯学部, 講師 (00305913)
TANIMURA Akihiko 北海道医療大学, 歯学部, 教授 (70217149)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 唾液腺 / シグナル伝達 / 唾液分泌 / アデノウイルス / 遺伝子導入 / GFP / カルシウム / 薬理学 |
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| Research Abstract |
Stim1-mKO1 was expressed in rat submandibular glands by retrograde ductal injection of an adenoviral vector. Stim1-mKO1 proteins were expressed predominantly in acinar cells following infection with an adenoviral vector. Confocal microscopy and Ca^ imaging analysis indicated that the Stim1-mKO1 expressed in submandibular acinar cells is functional. The overexpression of Stim1-mKO1 enhanced the agonist-induced Ca^ release from intracellular Ca^ stores and the Ca^ entry through store-operated Ca^ entry. It is possible that the overexpression of Stim1 increased salivary fluid secretion compared to the control. In addition, we succeeded in monitoring the agonist-induced Ca^ dynamics in SMG in live animals using the Ca^ indicator, G-GECO.
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