2012 Fiscal Year Final Research Report
Strategy of newly treatment for periodontal disease using the pathogenic bacterial genome information
| Project/Area Number |
22592078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 歯周病 / P.gingivalis / ゲノムプロジェクト / プロテアーゼ / マイナー線毛 / 抗体 |
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| Research Abstract |
Analyzing the genomic informationfor TDC60 is expected to provide new insights into the mechanisms underlying the onset and progression of periodontitis. For that reason, we searched for the specific virulence factor in the TDC60 strain using the complete genome sequence s,and found higher levels of adipeptidasePepD-2,a minorfibire-1(Mfa1),a collagenolysis-related peptidase Dap2 comparedto the W83 strain using 2-DE and MALD -TOF mass spectrometry. The crystal structure of PepD showed that the overall flding and dimeric organization. Bestatin has the ability to dose-dependentlyinhi it the P. gingivalis growth. Dipeptidases play a generalrole in the final break own of peptide fragments produced by other peptidases during the protein degradation process in P. gingivalis.
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Research Products
(23 results)
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[Journal Article] Identification of an O-antigen chain length regul tor, WzzP, in Porphyromonas gingivalis.2013
Author(s)
Shoji M, Yukitake H, Sato K, Shibata Y, Naito M, Aduse-Opoku J, Abiko Y, Curtis MA, N kayama K.
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Complete genome sequence of the bacterium Porphyr monas gingivalis TDC60, which causes periodontal disease.2011
Author(s)
Watanabe T, Maruyama F, Nozawa T, Aoki A, Okano S,Shibata Y, Oshima K, KurokawaK, Hattori M, Nakagawa I Abiko Y
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Journal Title
J Bacteriol
Volume: Vol193
Pages: 4259-4260
DOI
Peer Reviewed
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[Journal Article] Reynolds EC, Nakayama K, Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis :its cellular distribution,thioredoxin activity and role in heme utilization.2010
Author(s)
Mikio ShojiShibata Y, Shiroza T, Yukitake H, Peng B, Chen Y-Y, Sato K, Naito M, Abiko Y
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Journal Title
BMC Microbiol
Volume: Vol10
Pages: 152(12)
DOI
Peer Reviewed
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