2013 Fiscal Year Annual Research Report

自己組織化ナノテクノロジー技術を用いた細胞外マトリックスによる肥満細胞の制御

Research Project

Project/Area Number	22592099
Research Institution	Tohoku Fukushi University
Principal Investigator	杉本是明東北福祉大学, 総合福祉学部, 教授 (30361158)
Project Period (FY)	2010-04-01 – 2014-03-31
Keywords	再生医療 / ナノバイオ / バイオマテリアル / 肥満細胞 / 炎症 / アレルギー
Research Abstract	Mast cells are released from bone marrow into the circulatory system as immature precursors and differentiate upon their arrival at diverse organs and tissues. Because mast cell functions can be altered in these tissues, we propose that mast cells are sensitive to their surrounding microenvironment. To examine the morphological responses of mast cells, we cultured a proliferative mouse non-tumor cell line of mast cells (NCL-2 cells) on a honeycomb-like structured polystyrene film (HCF) representing a microenvironmental scaffold. In this study, the NCL-2 cells cultured on the HCF proliferated without apoptosis. Furthermore, NCL-2 cells cultured on 3- and 5-μm HCFs exhibited multinuclear formation. These observations of different NCL-2 cell morphologies and proliferation rates on HCF scaffolds with different hole sizes suggest that mast cells undertake specific proliferative shapes depending on the surrounding microenviroment. Moreover, HCFs may lead to the regulation of mast cell differentiation.
Current Status of Research Progress	Reason 25年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	25年度が最終年度であるため、記入しない。