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2012 Fiscal Year Final Research Report

Development of the bacteria origin protease activity and the genetic detection method using the electrochemical technique in endodontic treatment

Research Project

  • PDF
Project/Area Number 22592126
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionKyushu Dental College

Principal Investigator

NISHINO Takanobu  九州歯科大学, 歯学部, 歯学部 (80364157)

Co-Investigator(Kenkyū-buntansha) NAGAYOSHI Masato  九州歯科大学, 歯学部, 特別研修員 (30382419)
SATOU Shinobu  九州工業大学, 工学部, 准教授 (80510677)
KITAMURA Chiaki  九州歯科大学, 歯学部, 教授 (50265005)
NISHIHARA Tatsuji  九州歯科大学, 歯学部, 教授 (80192251)
Project Period (FY) 2010 – 2012
Keywords根管治療 / 細菌由来プロテアーゼ / 電気化学的検出
Research Abstract

In order to raise the success rate of endodontic treatment, it is necessary to precisely detect the bacteria which exist in a root canal or a periapical lesion. Therefore, a quick, simple, and positive bacteriological detection system at a chair side is strongly desired. In this study, detection of the bacteria by a fluorescence staining agent, and measurement by the electrochemical technique, were examined. Our results suggest possibility of a device to detect bacteria easily and precisely.

  • Research Products

    (13 results)

All 2013 2012 2011 2010

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (7 results)

  • [Journal Article] 教科書にみる歯内治療の科学的根拠と経験2013

    • Author(s)
      北村知昭,永吉雅人,西野宇信,鷲尾絢子 ,平田-土屋志津,市丸ー末松美希,吉居慎二,西藤法子,廉 クイ勲,中川愛加,中山 皓 平
    • Journal Title

      九州歯科学会雑誌

      Volume: 67(1) Pages: 1-4

    • Peer Reviewed
  • [Journal Article] Local regeneration of dentin-pulp complex using controlled release of FGF-2 and naturally derived sponge-like scaffolds.2012

    • Author(s)
      Kitamura C, Nishihara T, Terashita M, Tabata Y, Washio A
    • Journal Title

      International Journal of Dentistry

      Volume: volume2012 Pages: 8

    • DOI

      DOI:10.1155/2012/190561

    • Peer Reviewed
  • [Journal Article] Electrochemical Detection of Periodontal Disease Using Protease Assay2012

    • Author(s)
      Shinichiro Nagata, Takeshi Ohshima, Keiichi Ohtsuka, Shinobu Sato, Masato Nagayoshi, Chiaki Kitamura, Tatsuji Nishihara, & Shigeori Takenaka
    • Journal Title

      Peptide Science 2011

      Volume: 1 Pages: 407-408

    • Peer Reviewed
  • [Journal Article] Regeneration approach for dental pulp and periapical tissues with growth factors, biomaterials, and laser irradiation.2011

    • Author(s)
      Chiaki Kitamura, Tatsuji Nishihara, Masamichi Terashita, Yasuhiko Tabata, Eijiro Jimi, Ayako Washio and Shizu Hirata
    • Journal Title

      Polymers

      Volume: 3 Pages: 1776-1793

    • DOI

      DOI:10.3390/polym3041776

    • Peer Reviewed
  • [Journal Article] Bactericidal effects of diode laser irradiation on Enterococcus faecalis using periapical lesion defect model2011

    • Author(s)
      Masato Nagayoshi, Tatsuji Nishihara, Keisuke Nakashima, Shigetsugu Iwaki, Ker-Kong Chen, Masamichi Terashita, and Chiaki Kitamura
    • Journal Title

      ISRN Dentistry

      Pages: 6

    • DOI

      DOI:10.5402/2011/870364

    • Peer Reviewed
  • [Journal Article] 歯科保存学の現状と先端科学2010

    • Author(s)
      北村知昭, 西野宇信, 矢野淳也, 永吉雅人, 鷲尾絢子, 平田志津, 吉居慎二, 西藤法子
    • Journal Title

      九州歯科学会雑誌

      Volume: 64 Pages: 66-73

    • Peer Reviewed
  • [Presentation] 歯内治療における術後疼痛の分析-感染根管治療における術後疼痛の発生頻度に影響を与える因子の解析2012

    • Author(s)
      永吉雅人
    • Organizer
      第33回日本歯内療法学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      20120616-17
  • [Presentation] 歯内治療における術後疼痛の分析 -(1)根管治療中の術後疼痛の発生頻度と根尖孔穿通が術後疼痛出現に与える影響の解析2012

    • Author(s)
      永吉雅人
    • Organizer
      第72回九州歯科学会総会
    • Place of Presentation
      北九州
    • Year and Date
      20120519-20
  • [Presentation] 3D FEA of fiber post-core construction for endodonticallytreated teeth2012

    • Author(s)
      Nishino T
    • Organizer
      The Fifth Japan-Korea Joint Symposium on Bio-microsensing Technology
    • Place of Presentation
      北九州
    • Year and Date
      2012-10-26
  • [Presentation] フェロセン化ペプチド修飾電極を用いた歯周病関連酵素ジンジパインの活性検出2012

    • Author(s)
      島本隼平
    • Organizer
      日本分析化学会第610会
    • Place of Presentation
      金沢
    • Year and Date
      2012-09-21
  • [Presentation] 感染根管治療時の意図的根尖狭窄部拡大が術後疼痛発生に与える影響と非歯原性疼痛との関連の検討2011

    • Author(s)
      永吉雅人
    • Organizer
      日本歯科保存学会秋季学術大会
    • Place of Presentation
      大阪
    • Year and Date
      20111020-21
  • [Presentation] Quantification of Endodontic bacteria using Propidium Monoazide(PMA)2010

    • Author(s)
      NAGAYOSHI Masato
    • Organizer
      with Real-time PCR., Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      北九州
    • Year and Date
      20101120-21
  • [Presentation] Propidium monoazide(PMA)を用いたReal-time PCR法による根管内細菌の定量化の試み2010

    • Author(s)
      永吉雅人
    • Organizer
      日本歯科保存学会秋季学術大会
    • Place of Presentation
      岐阜
    • Year and Date
      20100128-29

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Published: 2014-08-29  

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