2012 Fiscal Year Final Research Report
Bacterial influence to the etiology of temporomandibular joint disorders;a cloning and sequencing analysis
| Project/Area Number |
22592236
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KANEYAMA Keiseki 金沢医科大学, 医学部, 講師 (50329380)
HONJO Miho 金沢医科大学, 医学部, 助教 (70424900)
ONO Toru 金沢医科大学, 医学部, 助教 (60297968)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 顎関節症 / 細菌感染 / 滑液 / 遺伝子解析 |
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| Research Abstract |
Universal bacterial primers were used to amplify 16S rRNA genes in 31 synovial fluid samples from 31 patients with TMD (TMD group). Subsequently, PCR amplicons were purified and cloned. Partial 16S rRNA sequences of the cloned insert were used to determine the species identities or closest relative by comparison with known sequences using GeneBank. In results, 20 of 31 samples (64.5%) from patients in the TMD group were identified with the presence of bacterial DNA by PCR. The 5 control samples from the nonTMD group were all negative in this study. In conclusion, a wide variety of bacteria, including some not previously reported associated with TMD, wereidentified in the synovial fluids from patients with TMD.
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