2012 Fiscal Year Final Research Report
Clinical establishment of novel nerve donor as an auto-grafting material. -Application of dental nerve-
| Project/Area Number |
22592251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ITOH Souichiro 東京医科歯科大学, 生体材料工学研究所, 研究員 (10242190)
WANG Wei 東京医科歯科大学, 生体材料工学研究所, 助教 (60451944)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 歯髄 / 神経移植 / 自家移植 |
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| Research Abstract |
This study was designed to examine whether a dental pulp could be a candidate of donor for nerve grafting in xenografting model. The dental pulp was obtained from a human vital extracted tooth, and treated with freezing and thawing method for reducing antigenicity. The treated sample was inserted into chitosan mesh tube, and then the complex was implanted into transected sciatic nerve in Sprague-Dawley (SD) rats. As early as 4 weeks after grafting, regenerating axons accompanied by host Schwann cells were found to grout out through basal laminae by electron microscopy. Twelve weeks after grafting, sporadic axonal regeneration was confirmed by light microscopy, thirty-two weeks after implantation, aggregation of axons was observed in this group. These findings clearly demonstrate that even dental pulp can act as conduits for regenerating axons.
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