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2012 Fiscal Year Final Research Report

MFCS4 in palate development

Research Project

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Project/Area Number 22592254
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

OKUHARA Shigeru  東京医科歯科大学, 大学院・医歯学総合研究科, 非常勤講師 (10451973)

Co-Investigator(Kenkyū-buntansha) SATO Yutaka  東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (90361716)
ISEKI Sachiko  東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (80251544)
Project Period (FY) 2010 – 2012
Keywords病態検査学 / 口蓋裂
Research Abstract

MFCS4 is one of cis-regulatory sequence for Shh transcription and its activation was found in oral and pharyngeal region from E11.00-E15.50. Compound deletion heterozygotes for Shh and MFCS4 show cleft palate. In palatal development, elevation of palatal shelves is achieved by corporation of intrinsic elevation force and change in shape and motion of the tongue. Because the palate of the mutant without tongue in organ culture successfully elevated and the exterior and histological preparation of the mutant tongue was hypomorphic, we focused on the relationship between tongue development and Shh signaling. In the tongue of the mutant, arrangement of the myotube was not organized or symmetric. Basal membrane of lingual epithelium that was discontinuous and without wave, and lingual septum that was again discontinuous and hypomorphic, were suggested to cause myotube disarrangement. As Shh signaling was previously reported to regulate the expression of laminin, that is a key component for basal membrane, and Sox9, that is a master transcription factor for tendon development, histological significances found in the tongue of the mutant agree with molecular mechanisms. These findings will be published with molecular evidences.

  • Research Products

    (4 results)

All 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Effects of embryonic hypoxia on lip formation.2012

    • Author(s)
      Nagaoka R, Okuhara S, Sato Y, Amagasa T, Iseki S.
    • Journal Title

      Birth Defects Res A Clin Mol Teratol.

      Volume: 94(4) Pages: 215-22

    • DOI

      doi:10.1002/bdra.23000

    • Peer Reviewed
  • [Journal Article] Pocket epithelium in the pathological setting for HMGB1 release.2011

    • Author(s)
      Ebe N, Hara-Yokoyama M, Iwasaki K, Iseki S, Okuhara S, Podyma-Inoue KA, Terasawa K, Watanabe A, Akizuki T, Watanabe H, Yanagishita M,Izumi Y.
    • Journal Title

      J Dent Res.

      Volume: 90(2) Pages: 235-40

    • DOI

      doi:10.1177/0022034510385688

    • Peer Reviewed
  • [Presentation] Tongue and palate development in Shh-/+MFCS4+/-2012

    • Author(s)
      Shigeru OKUHARA, Ryosuke NAGAOKA, Takanori AMANO, Tomoko SAGAI, Toshihiko SHIROISHI, Sachiko ISEKI
    • Organizer
      Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
    • Place of Presentation
      神戸
    • Year and Date
      2012-05-28
  • [Presentation] Tongue development in Shh-/+MFCS4+/-.2012

    • Author(s)
      Shigeru OKUHARA, Ryosuke NAGAOKA,Takanori AMANO, Tomoko SAGAI, ToshihikoSHIROISHI, Sachiko ISEKI
    • Organizer
      Gordon Research Conference Craniofacial Morphogenesis & Tissue Regeneration
    • Place of Presentation
      Ventura, CA, USA
    • Year and Date
      2012-03-18

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Published: 2014-08-29  

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