Screening for mRNAs responsible for morphogenesis in various lingual papillae.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22592266
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: YOSHIMURA Ken 日本歯科大学, 新潟生命歯学部, 准教授 (90297953)
• Co-Investigator(Kenkyū-buntansha): SHINDO Junji 北里大学, 獣医学部, 准教授 (80453512)
• Co-Investigator(Renkei-kenkyūsha): NASHIDA Tomoko 日本歯科大学, 新潟生命歯学部, 准教授 (10133464) ; IMAI Akane 日本歯科大学, 新潟生命歯学部, 准教授 (60180080) ; SHIMOMURA Hiromi 日本歯科大学, 新潟生命歯学部, 教授 (40139259) ; NAKAMURA Yasunori 日本歯科大学, 新潟生命歯学部, 准教授 (20095201) ; KATO Chihomi 日本歯科大学, 新潟生命歯学部, 講師 (00147860)
• Project Period (FY): 2010 – 2012
• Keywords: 舌粘膜新生技術 / マイクロアレイ / 舌乳頭形態形成 / ブタ / ラット

Research Abstract

To clarify comprehensive morphogenesis of the lingual papillae and establishment of their bio-engineered regeneration, we observed the histology of lingual mucosa and their lingual papillae during morphogenesis in porcine and rat embryos. Moreover, we analyzed about 22,000 mRNA lingual mucosa genes during the morphogenesis of rat embryos. Histological investigations revealed that there were some differences regarding the period of morphogenesis in porcine and rat embryos. This implies that there are some differences of morphogenesis among animal species. We also found that there was one clear fluctuation stage of mRNA expression during the rat morphogenesis. Some of these genes represented specific localizations on lingual tissues. These data would contribute to the identification of the morphogenesis-related genes to clarity the mechanism of the morphogenesis of lingual papillae.