2012 Fiscal Year Final Research Report

Development of multi-layered stem cell sheet transplant materialfor periodontal regenerative therapy

Research Project

Project/Area Number	22592315
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Periodontal dentistry
Research Institution	Nagasaki University
Principal Investigator	ABE Tatsuya 長崎大学, 大学院・医歯薬学総合研究科, 客員研究員 (80271112)
Co-Investigator(Kenkyū-buntansha)	HARA Yoshitaka 長崎大学, 大学院・医歯薬学総合研究科, 教授 (60159100)
Project Period (FY)	2010 – 2012
Keywords	移植・再生医療 / 再生医学 / 細胞・組織 / 歯学 / シクナル伝達
Research Abstract	In this study, we developed a multi-layered stem cell sheet transplant material with the aim to establish a new method for periodontal regenerative therapy. In the conventional culture medium, the proliferation of the mesenchymal stem cells stopped after forming confluent monolayer. However, the cells continued to grow and formed multi-layer sheets in the presence of TGF-β1 and ascorbic acid. Further addition ofROCK inhibitor Y-27632 to the medium containing TGF-β1 and ascorbic acid prevented the contraction of the cell sheets caused by TGF-β1. The cells in the multi-layered sheetsexpressed the same surface markers with those of the original mesenchymal stem cells and had the potential to differentiate into many cell lineages. These results indicated that this multi-layered stem cell sheet transplant material is useful for periodontal regenerative therapy.

(2 results)