2012 Fiscal Year Final Research Report
Osteopontin Level in Synovial Fluid is Associated with the Severity of Joint Pain and Cartilage Degeneration After Anterior Cruciate Ligament Rupture
Project/Area Number |
22600002
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
疼痛学
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
JYU Neishin 東京医科歯科大学, 医学部, 非常勤講師 (10436636)
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Co-Investigator(Kenkyū-buntansha) |
TSUJI Kunikazu 東京医科歯科大学, 歯と骨のGCOE拠点, 特任講師 (20323694)
MUNETA Takeshi 東京医科歯科大学, 医歯学総合研究科, 教授 (50190864)
SEKIYA Ichiro 東京医科歯科大学, 医歯学総合研究科, 教授 (10345291)
ASOU Yoshinori 東京医科歯科大学, 医歯学総合研究科, 寄付講座教員 (50345279)
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Project Period (FY) |
2010 – 2012
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Keywords | 変形性膝関節症 / Osteopontin |
Research Abstract |
Osteopontin (OPN) is an O-glycosylated phosphoprotein which is synthesized in a variety of tissues and cells including chondrocytes and synoviocytes. Accumulating data indicated that OPN is involved in the process of inflammation, immunity, and bone metabolism. OPN knockout mice have already been created and shown that cartilage degradation is accelerated in the absence of OPN. Human studies revealed that OPN protein level increased in the synovial fluid from the patients suffering from OA and RA. These data strongly suggest that OPN is involved in joint homeostasis and in the pathogenesis of arthritis. However, the molecular functions of OPN in these processes are not yet extensively studied. Here we report that synovial fluid OPN level is associated with the severity of joint pain.This study was approved by the Ethics Committee of this institute. All patients included in this study gave their full, written, informed consent for participation prior to the operative procedure. Tissue s
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amples (synovial fluid and synovial membrane) were obtained from the patients who underwent anterior cruciate ligament reconstruction (ACL-R) from January 2009 till October 2011 in our hospital. OPN mRNA expressed in synovial membrane was quantified by RT-QPCR (Roche, Light Cycler 480, Germany). Total OPN protein levels in synovial fluid were quantified using OPN/OPN N-half ELISA kit (IBL, Japan) and compared them with clinical parameters such as Lysholm score, visual analogue scale (VAS), serum C-reactive protein (CRP) level, and macroscopic observation of cartilage degradation.As previously reported, OPN mRNA expression level in synovial membrane and OPNprotein level in synovial fluid were significantly increased in OA if compared with those of ACL-R.In the ACL-R group, OPN protein level in synovial fluid was gradually decreased after the injury. We found that OPN protein level in synovial fluid was POSITIVELY associated with the severity of joint pain (VAS). Since OPN acts as a pro-inflammatory cytokines by enhancing migration, survival, phagocytosis, and pro-inflammatory cytokine production of macrophages, we hypothesized that OPN induces joint pain by promoting inflammation in the joint. To test this hypothesis, we investigated the correlation of OPN protein level in synovial fluid with serum CRP level. However, we did not observe any correlation between these two. However, OPN level was positively correlated with the volume of synovial fluid, a marker for joint inflammation. Less
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Research Products
(34 results)
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[Journal Article] Effect of posterolateral bundle graft fixation angles on graft tension curves and load sharing in double-bundle anterior cruciate ligament reconstruction using a transtibial drilling technique.2013
Author(s)
Koga, H., Muneta, T., Yagishita, K., Ju, Y.J., Mochizuki, T., Horie, M., Nakamura, T., Okawa, A., and Sekiya, I.
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Journal Title
Arthroscopy
Volume: 29
Pages: 529-538
Peer Reviewed
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[Journal Article] Remnant volume of anterior cruciate ligament correlates preoperative patients' status and postoperative outcome.2013
Author(s)
Muneta, T., Koga, H., Ju, Y.J., Horie, M., Nakamura, T., and Sekiya, I.
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Journal Title
Knee Surg Sports Traumatol Arthrosc
Volume: 21
Pages: 906-913
Peer Reviewed
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[Journal Article] The kinematic analysis of female subjects after double-bundle anterior cruciate ligament reconstruction during single-leg squatting.2013
Author(s)
Yamazaki, J., Muneta, T., Ju, Y.J., Koga, H., Morito, T., and Sekiya, I.
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Journal Title
J Orthop Sci
Volume: 18
Pages: 284-289
Peer Reviewed
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[Journal Article] High-flex posterior cruciate-retaining vs posterior cruciate-substituting designs in simultaneous bilateral total knee arthroplasty: a prospective, randomized study.2012
Author(s)
Yagishita, K., Muneta, T., Ju, Y.J., Morito, T., Yamazaki, J., and Sekiya, I.
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Journal Title
J Arthroplasty
Volume: 27
Pages: 368-374
Peer Reviewed
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[Journal Article] Osteopontin level in synovial fluid is associated with the severity of joint pain and cartilage degradation afteranterior cruciate ligament rupture.2012
Author(s)
Yamaga, M., Tsuji, K., Miyatake, K., Yamada, J., Abula, K., Ju, Y.J., Sekiya, I., and Muneta, T.
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Journal Title
PLoS One
Volume: 7
Pages: e49014
Peer Reviewed
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[Journal Article] Hip acetabular dysplasia and joint laxity of female anterior cruciate ligament-injured patients.2011
Author(s)
Yamazaki, J., Muneta, T., Ju, Y.J., Morito, T., Okuwaki, T., and Sekiya, I.
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Journal Title
Am J Sports Med
Volume: 39
Pages: 410-414
Peer Reviewed
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[Journal Article] Radiographic description of femoral tunnel placement expressed asintercondylar clock time in double-bundle anterior cruciate ligament reconstruction.2011
Author(s)
Yamazaki, J., Muneta, T., Koga, H., Sekiya, I., Ju, Y.J., Morito, T., and Yagishita, K.
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Journal Title
Knee Surg Sports Traumatol Arthrosc
Volume: 19
Pages: 418-423
Peer Reviewed
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[Presentation] BMP-7 treated Achilles tendon transplantation for meniscal defect in a rat model.2012
Author(s)
Ozeki N., Sekiya I., Otabe K., Katagiri H., Okuno M., Tsuji K., Saito T., and Muneta T.
Organizer
Orthopaedic Research Society
Place of Presentation
Moscone West Convention Center, San Francisco,CA, USA
Year and Date
20120204-07
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[Presentation] Syngenic, minor mismatched, and major mismatched transplantation of synovial mesenchymal stem cells in a rat massive meniscal defect model.2012
Author(s)
Okuno M., Sekiya I., Otabe K., Katagigi H., Ozeki N., Horie M., Koga H., Tsuji K., Yoshiya S., and Muneta T.
Organizer
Orthopaedic Research Society.
Place of Presentation
Moscone West Convention Center, San Francisco, CA, USA
Year and Date
20120204-07
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[Presentation] Isolation and characterization of multi-potential mesenchymal cells from mouse synovium.2011
Author(s)
Futami I., Ishijima M., Kaneko H., Tsuji K., Tomikawa NI., Sekiya I., Muneta T., Hirasawa EA., and Kaneko K.
Organizer
World Congress on Osteoarthritis (OARSI)
Place of Presentation
Hilton Bay Front, SanDiego, CA, USA
Year and Date
20110915-18
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