2012 Fiscal Year Final Research Report
Development of RNA aptamer against toxic Aβ oligomer
| Project/Area Number |
22603006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
ケミカルバイオロジー
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| Research Institution | Kyoto University |
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Principal Investigator |
MURAKAMI Kazuma 京都大学, 大学院・農学研究科, 助教 (80571281)
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| Research Collaborator |
SHIMIZU Takahiko 千葉大学, 大学院・医学研究院, 客員准教授 (40301791)
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| Project Period (FY) |
2010 – 2012
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| Keywords | アルツハイマー病 / アミロイド β / 凝集 / オリゴマー / アプタマー / RNA |
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| Research Abstract |
Amyloid β-protein (Aβ), a causative agent of Alzheimer’s disease, aggregates and shows neurotoxicity. It has been well documented that Aβ oligomer (toxic dimer) is mostly related to the neurotoxicity. We have demonstrated that the formation of turn structure at position 22 and 23 plays a critical role in the oligomerization of Aβ. However, it is quite difficult to synthesize Aβoligomer due to the potent ability of Aβ to aggregate. To develop RNA apatamer against toxic Aβdimer, we synthesized Aβ40 dimer, in which Ala30 was substituted with diaminopimelic used as anintermolecular linker. The development of aptamer against Aβ40 dimer is under way.
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