2012 Fiscal Year Final Research Report
Studies on microbial secondary metabolism for development of biosynthetic technology tools
| Project/Area Number |
22603011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
ケミカルバイオロジー
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 生合成 / チオアミド / メチレン架橋 / thioviridamide / hatomarubigin |
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| Research Abstract |
The genome analysis of a thioviridamide-producing organism revealed a gene encoding the thioviridamide precursor peptide (tvaA). Heterologous production of thioviridamide by 15 genes (tvaA- tvaO) identified the thioviridamide biosynthetic gene cluster including thioamide-forming genes. In the hatomarubigin biosynthetic gene cluster, hrbYand hrbUwere identified as a methylene-bridge forming gene and a methyltransferase gene, respectively. The hrbF-deficient gene cluster produced 5-hydroxyhatomarubigin E, suggesting that hrbFcontrols the hydroxylation regioselectivity.
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Research Products
(9 results)
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[Journal Article] Biverlactones A-D, new circumventors of arbekacin resistance in MRSA2011
Author(s)
Masato Iwatsuki, Toshiya Ishimori, Tsuyoshi Yamamoto, Keiko Takata, Mihoko Mori, Kenichi Nonaka, Rokuro Masuma, Yoichi Hayakawa, Hideaki Hanaki, Kazuro Shiomi, Satoshi Omura
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Journal Title
Penicillium sp
Volume: 67
Pages: 6644-6648
Peer Reviewed
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