2012 Fiscal Year Final Research Report
Detection and observation of oxidative damage for the improvement of high-LET radiation therapy
| Project/Area Number |
22611015
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical Physics/Radiological Technology
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| Research Institution | Tokai University |
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Principal Investigator |
ITO Atsushi 東海大学, 工学部, 教授 (80193473)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Naoaki 東海大学, 医学部, 教授 (60096196)
HIRAYAMA Ryoichi 独立行政法人放射線医学総合研究所, 重粒子医科学センター, 研究員 (90435701)
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| Co-Investigator(Renkei-kenkyūsha) |
MURAYAMA Chieko 東海大学, 医学部, 講師 (50307295)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 高LET放射線 / 間接作用 / ラジカル / 重粒子 / DNA酸化損傷 / 免疫染色法 / 8-OHdG / 酸素効果 / トラック構造 / DNA薄膜 |
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| Research Abstract |
In order to visualize the distribution of a penumbra, a low LET region around ion tracks, we have developed the methods to image the distribution of 8-OHdG, a radiation product resulting from the reaction of OH radicals with guanine residue, using an immunofluorescence staining for 1) mouse brain tissue, 2) human A549 cells, and 3) DNA thin film. Positive stains were evident particularly in the peripheral region of the cerebrum and in Purkinje cells of the cerebellum in mice. The immunofluorescence staining of cell nuclei was detected at a dose as small as 5 Gy where we can distinguish each incoming particle. DNA film with an insoluble property to water was developed with special reference to the uniformity, and the treatment with Fenton reaction to generate OH radicals resulted in the successful staining of uniformly formed 8-OHdG.
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[Journal Article] Furusawa: Induction of DNA DSB and its rejoining in clamped and non-clamped tumors after exposure to carbon ion beams in comparison to X-rays2011
Author(s)
R. Hirayama, A. Uzawa, Y. Matsumoto, M. Noguchi, Y. Kase, N. Takase, A. Ito, S. Koike, K. Ando, R. Okayasu, Y
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Journal Title
Radiat.Prot. Dosimetry
Volume: 143
Pages: 508-512
Peer Reviewed
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[Journal Article] Direct and indirect actions to high-LET radiations2010
Author(s)
R. Hirayama, Y. Matsumoto, Y. Kase, M.Noguchi, N. Takase, A. Uzawa, A. Ito,N. Matsufuji, K. Ando, R. Okayasu, Y.Furusawa
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Journal Title
NIRS-KI Joint Symposium on Ion-Radiation Sciences
Volume: 235
Pages: 155-162
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