2012 Fiscal Year Final Research Report
Study of designer cellulosome for complete saccharification of soft-biomass
| Project/Area Number |
22612001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomass energy
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| Research Institution | Mie University |
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Principal Investigator |
MIYAKE Hideo 三重大学, 大学院・生物資源学研究科, 助教 (50362364)
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| Co-Investigator(Renkei-kenkyūsha) |
KURODA Kouichi 京都大学, 大学院・農学研究科, 准教授 (30432339)
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| Project Period (FY) |
2010 – 2012
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| Keywords | cellulosome / Clostridium cellulovoran / proteome |
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| Research Abstract |
Proteome analysis of the Clostridium cellulovorans after culture in various carbon sources demonstrated to find enzymes which are necessary to reconstruct a cellulosome. Not only several carbohydrate enzymes but also proteins of unknown function were detected. Genomic DNA coding for scaffolding protein cbpB and 10 cellulosomal enzymes were detected by proteome analysis were subcloned in order to express and produce the recombinant protein to reconstruct a cellulosome.
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Research Products
(14 results)
