2011 Fiscal Year Final Research Report
Analysis of a gene function using ZFN and recombineering
| Project/Area Number |
22650079
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Kyorin University (2011)
Tokyo Metropolitan Organization for Medical Research (2010) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | ZFN / recombineering / CaMKII / dNZF |
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| Research Abstract |
A method for studying a gene function with complete control has been tried to develop by combinatorial use of a'zinc finger nuclease'(ZFN), which is a designed, sequence-specific endonuclease that can be customized to cleave a user-chosen DNA target, and recombineering, which is able to engineer large segments of genomic DNA of P[acman] for transgenesis in Drosophila melanogaster.
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