In vivo imaging of sensory synaptic inputs in neocortex

2011 Fiscal Year Final Research Report

• Project/Area Number: 22650083
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Fusional basic brain science
• Research Institution: The University of Tokyo
• Principal Investigator: KITAMURA Kazuo 東京大学, 大学院・医学系研究科, 准教授 (60423159)
• Project Period (FY): 2010 – 2011
• Keywords: ニューロン / シナプス / 2光子イメージング / シナプス統合 / 樹状突起

Research Abstract

We performed simultaneous whole-cell patch-clamp recordings and two-photon calcium imaging in layer 2/3 of mouse barrel cortex in vivo. Calcium signals in individual spines of layer 2/3 pyramidal cells induced by spontaneous and sensory-evoked activities could be clearly observed. We found that approximately 80% of synaptic inputs were localized only in 25% of total spines. Furthermore, we found that nearby spines were tended to be activated simultaneously. Thus, our results suggest that functionally coupled neurons innervate to nearby spines in dendrites of postsynaptic pyramidal neurons.

Research Products

• [Journal Article] 小脳プルキンエ細胞樹状突起活動のin vivoイメージング (2012)
  • Author(s): 喜多村和郎, 橋本浩一, 狩野方伸
  • Journal Title: 生体の科学 Volume: 63 Pages: 26-33
• [Journal Article] Locally synchronized synaptic inputs (2012)
  • Author(s): Takahashi, N., Kitamura, K., Matsuo, N., Mayford, M., Kano, M., Matsuki, N. & Ikegaya, Y
  • Journal Title: Science Volume: 335 Pages: 353-356
  • DOI: DOI:10.1126/science.1210362
  • Peer Reviewed
• [Journal Article] Dendritic calcium signaling triggered by spontaneous and sensory-evoked climbing fiber input to cerebellar Purkinje cells in vivo (2011)
  • Author(s): Kitamura, K. & Hausser, M
  • Journal Title: J. Neurosci Volume: 31 Pages: 10847-10858
  • DOI: DOI:10.1523/JNEUROSCI.2525-10.2011
  • Peer Reviewed
• [Journal Article] Postsynaptic P/Q-type Ca2+channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum (2011)
  • Author(s): Hashimoto, K., Tsujita, M., Miyazaki, T., Kitamura, K., Yamazaki, M., Shin, H.-S., Watanabe, M., Sakimura, K. & Kano M
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 9987-9992
  • DOI: DOI:10.1073/pnas.1101488108
  • Peer Reviewed
• [Journal Article] In vivo two-photon uncaging of glutamate revealing the structure-function relationships of dendritic spines in the neocortex of adult mice (2011)
  • Author(s): Noguchi, J., Nagaoka, A., Watanabe, S., Ellis-Davies, G. C., Kitamura, K., Kano, M., Matsuzaki, M. & Kasai, H.
  • Journal Title: J. Physiol Volume: 589 Pages: 2447-2457
  • DOI: DOI:10.1113/jphysiol.2011.207100
  • Peer Reviewed
• [Presentation] Two-photon imaging of neuronal population activities in cerebellar cortex (2011)
  • Author(s): Kazuo Kitamura
  • Organizer: 分子生物学会(ワークショップ)
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] Heterogeneous organization of sensory synaptic inputs in the mouse barrel cortex (2011)
  • Author(s): Kazuo Kitamura
  • Organizer: 生理研国際研究集会
  • Place of Presentation: 岡崎カンファレンスセンター(愛知県)
  • Year and Date: 2011-12-09
• [Presentation] Enhanced synchrony of climbing fiber-induced Ca2+signaling in cerebellar Purkinje cells caused by elevated synchronous activity of inferior olivary neurons in GluD2(GluRδ2) knockout mouse (2011)
  • Author(s): Miki Hashizume, Kazuo, Kitamura, Kenji Sakimura and Masanobu Kano
  • Organizer: Neuro2011
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-14
• [Presentation] Desynchronization of cerebellar Purkinje cell population activity during postnatal development (2011)
  • Author(s): Jean-Marc Good, Kazuo Kitamura, Masanobu Kano
  • Organizer: Neuro2011
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-14
• [Presentation] Two-photon imaging of Purkinje cell dendritic activity in vivo (2011)
  • Author(s): Kazuo Kitamura
  • Organizer: IBRO World Congress(シンポジウム)
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-07-16
• [Presentation] Dendritic activities of cerebellar Purkinje cell in vivo (2011)
  • Author(s): K. Kitamura & M. Hausser
  • Organizer: 第88回日本生理学大会
  • Place of Presentation: 誌上開催
  • Year and Date: 2011-03-28
• [Presentation] 登上線維の発達過程におけるP/Q型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本浩一、辻田美加、宮崎太輔、喜多村和郎、山崎真弥、Shin, H.-S.、渡辺雅彦、崎村建司、狩野方伸
  • Organizer: Neuro2010(シンポジウム)
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-04
• [Presentation] GluD2(GluRδ2)ノックアウトマウスの小脳プルキンエ細胞集団の2光子カルシウムイメージング解析 (2010)
  • Author(s): 橋爪幹、喜多村和郎、崎村建司、狩野方伸
  • Organizer: Neuro2010
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-02
• [Presentation] Simultaneous two-photon calcium imaging and patch-clamp recordings in vivo (2010)
  • Author(s): K. Kitamura, M. Hausser & M. Kano
  • Organizer: Jacques Monod Conference "Imaging brain circuits in health and disease"
  • Place of Presentation: Roscoff, France
  • Year and Date: 2010-07-01
• [Presentation] 生体内における神経活動の2光子イメージング (2010)
  • Author(s): 喜多村和郎
  • Organizer: 第5回日本分子イメージング学会総会・学術集会
  • Place of Presentation: ピアザ淡海(滋賀県)
  • Year and Date: 2010-05-22
• [Presentation] 小脳登上線維。プルキンエ細胞シナプスの生後発達におけるP/Q型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本浩一、辻田美加、宮崎太輔、喜多村和郎、山崎真弥、Shin, H.-S.、渡辺雅彦、崎村建司、狩野方伸
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手県)
  • Year and Date: 2010-05-19
• [Book] 第12章in vivoイメージングパッチ法(最新パッチクランプ実験技術法、岡田泰伸編) (2011)
  • Author(s): 喜多村和郎
  • Total Pages: 121-128
  • Publisher: 吉岡書店