2011 Fiscal Year Final Research Report
Exploring Optical Navigation of Neurite Outgrowth
| Project/Area Number |
22650084
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Fusional basic brain science
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISEKI Mineo 東邦大学, 薬学部, 准教授 (60414009)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 光脳科学 / 神経突起 / 神経再生 |
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| Research Abstract |
To explore the optical navigation of neurite outgrowth, I utilized two photoreceptors : bacterial photoactivated adenylyl cyclase(bPAC) and an anonymous photoreceptor X. Transient expression of bPAC in cultured cells showed A-kinase activation upon light stimulation. I designed a fusion protein X-Trk, X-photoreceptor connected with the kinase domain of TrkB. Photostimulation upon the cells expressing X-Trk induced autophosphorylation of X-Trk and MAP kinase activation. Ectopic expression of X-Trk in chick embryonic dorsal root ganglion neurons facilitated the neurite outgrowth upon light stimulation. Enabling the optical navigation of neurite outgrowth with X-Trk may open new avenue to regenerate and analyze neural circuits.
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Research Products
(5 results)
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[Journal Article] Decreased Expression of Semaphorin-3A, a Neurite-Collapsing Factor, is Associated With Itch in Psoriatic Skin2012
Author(s)
Kou K, Nakamura F, Aihara M, Chen H, Seto K, Komori JY, Kambara T, Nagashima Y, Goshima Y, Ikezawa Z.
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Cartilage acidic protein-1B(LOTUS), an endogenous Nogo receptor antagonist for axon tract formation2011
Author(s)
Sato Y, Iketani M, Kurihara KYamaguchi M, Yamashita N, Nakamura F, Aire Y, Kawasaki T, Hirata T, Abe T, Kiyonari H, Strittmatter SM, Goshima Y, Takei T.
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Journal Title
Science
Volume: 333
Pages: 769-73
Peer Reviewed
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