2011 Fiscal Year Final Research Report
Development of a novel protein delivery system using peroxisomes
| Project/Area Number |
22650111
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUZAKI Takashi 大阪大学, 医学研究科, 特任研究員 (90456939)
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| Project Period (FY) |
2010 – 2011
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| Keywords | タンパク質送達システム / 細胞生物学 / ペルオキシソーム |
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| Research Abstract |
In this study, we developed a novel protein delivery system using peroxisome and the peroxisomal protein import system as a protein delivery carrier and a protein concentrator, respectively. First, we established an affinity purification method for highly purified peroxisomes in which the target protein had been efficiently imported intracellularly. Next, we developed an efficient method to display specific antibodies onto the surface of the purified peroxisomes and utilized it to an antibody-dependent and functional delivery of the target protein encapsulated in the peroxisomes. Finally, we successfully demonstrated that α-galactosidase A, a therapeutic protein agent for Fabry disease, could be efficiently delivered into the target lysosomes by using this novel protein delivery system.
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