2011 Fiscal Year Final Research Report
Study on three-dimensional arrangement control of an artificial extracellular matrix by imprinting cell surface morphology
| Project/Area Number |
22650112
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
KOBAYASHI Hisatoshi 独立行政法人物質・材料研究機構, 生体機能材料ユニット, グループリーダー (90354266)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIKAWA Chiaki 物質・材料研究機構, 生体機能材料ユニット, 研究員 (10447930)
TERADA Dohiko 物質・材料研究機構, 生体機能材料ユニット, ポスドク研究員 (10454555)
HATTORI Shinya 物質・材料研究機構, 生体機能材料ユニット, ポスドク研究員 (30469762)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 細胞・組織工学 / 高次構造組織化 |
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| Research Abstract |
The mechanism for generating the superstructure of corneal stroma was investigated. In a part of the generating the superstructure, we are hypothesizing as follows, the cells rearrange the ECM and play a role as the mold of the superstructure. To prove the hypothesis, we developed the new artificial ECM model using electrospun nanofibers and combined with fluid type culture system to give the condition similar to the tissue generation. In this system, the cell and the artificial ECM seems to be gotten the tensioning force by the medium flow, it's mimicking the force that created by the expansion of the tissue in the process of tissue generation. Under the condition, many cells bundled the nanofibers up and rearranged the ECM direction. A part of the structure looks similar to the natural corneal superstructure. We believe this system will be powerful tool to investigate more detail mechanism to create the superstructure.
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