2012 Fiscal Year Final Research Report
Gene transfer system into mesenchymal stem cell by peptide-modified liposomes and ultrasound technology
| Project/Area Number |
22650119
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical systems
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ARAMAKI Yukihiko 東京薬科大学, 薬学部, 教授 (90138959)
TAKAHASHI Yoko 東京薬科大学, 薬学部, 助手 (30453806)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAGI Norio 東京薬科大学, 薬学部, 准教授 (50318193)
MARUYAMA Kazuo 帝京大学, 薬学部, 教授 (30130040)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ペプチド修飾リポソーム / 超音波照射 / 遺伝子細胞療法 |
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| Research Abstract |
In this study, to enhance transfection efficiency by promoting the escape of the liposomes from the endosome to the cytosol and the nuclear localization of plasmid DNA, nuclear localization signals (NLS) peptides were used for condensing plasmid DNA encoding luciferase (pCMV-Luc) and embeding it into AG73-PEG liposomes. As a result, AG73-PEG liposomes showed selective gene delivery to cellsvia syndecan-2. Efficient gene transfection was observed. The efficacy was further enhanced in the combination of Bubble liposomes and US exposure. Thus, the combination of AG73-PEG liposomes with NLS peptides and BLs and US may be useful method to achieve selective and efficient gene delivery. This method may be also expected to a gene transfection system to mesenchymal stem cell in application of ex vivo non-viral gene therapy
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