2011 Fiscal Year Final Research Report
PHYSIOLOGICAL FUNCTIONS OF BRANCHED-CHAIN AMINO ACIDS IN MUSCLES
| Project/Area Number |
22650152
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Sports science
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KITAURA Yasuyuki 名古屋大学, 大学院・生命農学研究科, 助教 (90442954)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 分岐鎖アミノ酸(BCAA) / 分岐鎖α一ケト酸脱水素酵素(BCKDH) / BCKDHキナーゼ / 筋肉 / コンディショナルBDK-KOマウス / BCAA不足 / キメラマウス |
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| Research Abstract |
Branched-chainα-etoacid dehydrogenase(BCKDH) complex is a rate-limiting enzyme of the branched-chain amino acid(BCAA) catabolism and is regulated by the specific kinase(BCKDH kinase : BDK). In the present study, we aimed to generate muscle-specific BDK deficient mice, which may result in muscle-specific BCAA deficiency due to the acceleration of BCAA catabolism. We used the Cre-loxP system to generate the conditional BDK-knockout(KO) mice ; recombinant ES cells containing a conditional allele of the mouse BDK gene that was engineered by flanking exons 9-12 with loxP sites. Thus, exons 9-12 are deleted by the expression of Cre recombinase. In the first trial, we obtained chimeric mice without the floxed BDK gene in the germ line. In the second trial, we again obtained the chimeric mice, which is used to produce heterozygous BDK-gene-floxed offsprings.
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