Development of a gene amplification technique with localized heating of magnetic nanoparticles via application of alternating magnetic field

2011 Fiscal Year Final Research Report

• Project/Area Number: 22651046
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: ARAKAKI Atsushi 東京農工大学, 大学院・工学研究院, 准教授 (10367154)
• Project Period (FY): 2010 – 2011
• Keywords: 磁気微粒子 / 遺伝子増幅 / 誘導加熱 / 核酸回収 / 細胞破砕

Research Abstract

The aim of this research is development of a technique for gene amplification by localized heating of magnetic nanoparticles. We have applied the localized heating phenomena of magnetic nanoparticles to control the nucleicacid dissociation. In addition, the amine-modified magnetic particles have been demonstrated to inactive the microbe via the damage of the microbial membrane. Therefore, we propose to develop a nano-scale high throughput nucleic acid amplification technique further application in micro devices.

Research Products

• [Journal Article] Efficient DNA release from PAMAM dendrimer-modified superparamagnetic nanoparticles for DNA recovery. (2012)
  • Author(s): Atsushi Arakaki, Keiyu Shibata, Takeyuki Mogi, Masahito Hosokawa, Keiichi Hatakeyama, Hideyuki Gomyo, Tomoyuki Taguchi, Hitoshi Wake, Takeo Tanaami, Tadashi Matsunaga and Tsuyoshi Tanaka
  • Journal Title: Polymer Journal Volume: (in press)
  • DOI: DOI:10.1038/pj.2012.32
  • Peer Reviewed
• [Journal Article] Characterization of magnetic nanoparticles modified with thiol core, functionalized PAMAM dendron for DNA recovery (2012)
  • Author(s): Tsuyoshi Tanaka, Keiyu Shibata, Masahito Hosokawa, Keiichi Hatakeyama, Atsushi Arakaki, Hideyuki Gomyo, Takeyuki Mogi, Tomoyuki Taguchi, Hitoshi Wake, Takeo Tanaami, Tadashi Matsunaga
  • Journal Title: J. Colloid Interface Sci Volume: 377 Pages: 469-475
  • DOI: DOI:10.1016/j.jcis.2012.03.039
  • Peer Reviewed
• [Presentation] アミノ基修飾ナノ粒子の局所加熱を用いた微生物不活化法の開発 (2012)
  • Author(s): 高橋真美、細川正人、新垣篤史、田中剛、松永是
  • Organizer: 電気化学会第79回大会
  • Place of Presentation: アクトシティ浜松(静岡県)
  • Year and Date: 2012-03-30
• [Presentation] Development of influence virus gene detection technique based on located heating of magnetic nanoparticles (2011)
  • Author(s): 高橋真美、新垣篤史、田中剛、松永是
  • Organizer: 62^<nd> Annual Meeting of the International Society of Electrochemistry
  • Place of Presentation: 朱鷺メッセ(新潟県)
  • Year and Date: 2011-09-12
• [Presentation] 磁気微粒子の局所加熱による微生物の不活化条件の検討 (2011)
  • Author(s): 高橋真美、細川正人、新垣篤史、田中剛、松永是
  • Organizer: 2011年電気化学会秋季大会
  • Place of Presentation: 朱鷺メッセ(新潟県)
  • Year and Date: 2011-09-09
• [Remarks]
  • URL: http://www.tuat.ac.jp/~biomol/index.html