2011 Fiscal Year Final Research Report
Realization of a PCR-free, highly-sensitive DNA sensor
| Project/Area Number |
22655022
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
MIZUTANI Fumio 兵庫県立大学, 大学院・物質理学研究科, 教授 (80118603)
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| Co-Investigator(Renkei-kenkyūsha) |
IIJIMA Seiichiro (00356403)
NIWA Osamu (70392644)
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| Project Period (FY) |
2010 – 2011
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| Keywords | バイオセンサー / DNA / 高感度測定 / PCR-フリー |
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| Research Abstract |
A novel electrochemical technique for ds-DNA detection has been developed by combining chloro(2, 2': 6', 2''-terpyridine) Pt(II) and a glassy carbon electrode. The intercalation of the Pt(II) complex into ds-DNA caused suppression of the electroreduction of the Pt(II) complex owing to the steric hindrance, and subsequently, the electroreductive current for proton that is catalyzed by the platinum deposited on the electrode by the reduction of the Pt(II) complex. A target DNA content was determined from the decrease in the catalytic activity of the electrode for reducing proton in the presence of probe DNA.
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