Development about photo-triggered autonomous DNA computing

2011 Fiscal Year Final Research Report

• Project/Area Number: 22655051
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: FUJIMOTO Kenzo 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 教授 (90293894)
• Project Period (FY): 2010 – 2011
• Keywords: DNA / 光ライゲーション / 光クロスリンク

Research Abstract

We demonstrate that normal K-ras gene suppressed selectively by photo-triggered autonomous DNA computing and calculated survival rate by real time clamp PCR. BxPC-3 and Capan-1 were suppressed by photo-triggered autonomous DNA Ccamp respectively 86%, 47%.

Research Products

• [Journal Article] Stabilization of DNA nanostructures by photo-cross-linking (2011)
  • Author(s): Miho Tagawa, Koh-ichiroh Shohda, Kenzo Fujimoto and Akira Suyama
  • Journal Title: Soft Matter Volume: 7 Pages: 10931-10934
  • Peer Reviewed
• [Journal Article] Construction of branched DNA for SNP determination on glass-chip using photochemical ligation (2011)
  • Author(s): Minhaz Uddin Ahmed, Yoshinaga Yoshimura, M. Mosharraf Hossain, Eiichi Tamiya and Kenzo Fujimoto
  • Journal Title: Bio Chip J. Volume: 5 Pages: 206-221
  • Peer Reviewed
• [Journal Article] Signal turn-on probe for nucleic acid detection based on 19F nuclear magnetic resonance (2011)
  • Author(s): Takashi Sakamoto, Yu-ki Shimizu, Jun Sasaki, Hikaru Hayakawa and Kenzo Fujimoto
  • Journal Title: Bioorg Med. Chem. Lett Volume: 21 Pages: 303-206
  • Peer Reviewed
• [Journal Article] Site-Specific Photochemical RNA Editing (2010)
  • Author(s): Kenzo Fujimoto, Kaoru Konishi-Hiratsuka, Takashi Sakamoto and Yoshinaga Yoshimura
  • Journal Title: Chemical Communications Volume: 46 Pages: 7545-7548
  • Peer Reviewed
• [Journal Article] The Site-Specific Cytosineto the Uracil Transition Using Reversible DNA Photocrosslinking (2010)
  • Author(s): Kenzo Fujimoto, Takashi Sakamoto, Kaoru Konishi-Hiratsuka and Yoshinaga Yoshimura
  • Journal Title: Chem Bio Chem Volume: 11 Pages: 1661-1664
  • Peer Reviewed
• [Presentation] D Development of Photochemical DNA and RNA Manipulation (2011)
  • Author(s): Fujimoto Kenzo
  • Organizer: 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico YOKOHAMA(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] 酵素を使わずに化学の力でDNAを操る (2011)
  • Author(s): 藤本健造
  • Organizer: 日本化学会東北支部主催会員増強のための講演会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2011-11-25
• [Presentation] Development of Photo triggered DNA and RNA Manipulation (2011)
  • Author(s): Fujimoto Kenzo
  • Organizer: 5th Pacific Symposium on Radical Chemistry
  • Place of Presentation: Shirahama,(和歌山県)
  • Year and Date: 2011-09-27
• [Presentation] Photochemical DNA manipulation toward for DNA computing (2011)
  • Author(s): Nakamura Shigetaka, Ogasakawa Shinzi and Fujimoto Kenzo
  • Organizer: 17th International Conference on DNA Computing and Molecular Programming
  • Place of Presentation: LA, USA
  • Year and Date: 2011-09-22
• [Presentation] 光化学的な新規遺伝子操作法の開発とその応用 (2011)
  • Author(s): 藤本健造
  • Organizer: 日本化学会中国四国支部愛媛地区化学講演会
  • Place of Presentation: 愛媛大学(愛媛県)
  • Year and Date: 2011-09-14