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2011 Fiscal Year Final Research Report

On-demand and non-invasive cell detachment technology

Research Project

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Project/Area Number 22656187
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionUniversity of Tsukuba

Principal Investigator

FUKUDA Junji  筑波大学, 数理物質系, 講師 (80431675)

Co-Investigator(Kenkyū-buntansha) SUZUKI Hiroaki  筑波大学, 数理物質系, 教授 (20282337)
Project Period (FY) 2010 – 2011
Keywords医用化学工学 / 動物細胞培養 / 自己組織化単分子膜 / 透明電極 / バイオ関連機器 / 細胞チップ / マイクロアレイ
Research Abstract

The aim of this project is to develop a technology for detaching specific cells being observed under a microscopy with single-cell resolution. We first demonstrated that cells on a transparent electrode (ITO electrode) can be rapidly detached by using an electrochemical reaction. We subsequently fabricated a microarray of the electrode so that an electrical potential can be separately applied to the electrodes. This electrode system can be used to detach individual cells one by one.

  • Research Products

    (33 results)

All 2012 2011 2010 Other

All Journal Article (8 results) Presentation (23 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Tissue Engineering Based on Electrochemical Desorption of an RGD-Containing Oligopeptide2012

    • Author(s)
      N. Mochizuki, T. Kakegawa, T. Osaki, N. Sadr, NN. Kachouie, H. Suzuki, J. Fukuda
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine (IF=3.53)

    • DOI

      DOI:10.1002/term.519

  • [Journal Article] On-chip diagnosis of subclinical mastitis in cows by electrochemical measurement of neutrophil activity in milk2012

    • Author(s)
      S. Kimura, J. Fukuda, A. Tajima and H. Suzuki
    • Journal Title

      Lab on a Chip (IF=6.26)

      Volume: 12 (7) Pages: 1309-15

    • DOI

      DOI:10.1039/C2LC20952G

  • [Journal Article] Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis2011

    • Author(s)
      C. H. Kwon, I. Wheeldon, N. N. Kachouie, S. H. Lee, H. Bae, S. Sant, J. Fukuda, J. W. Kang, and A. Khademhosseini
    • Journal Title

      Analytical Chemistry (IF=5.87)

      Volume: 83 (11) Pages: 4118-25

    • DOI

      DOI:10.1021/ac200267t

  • [Journal Article] Spatio-temporal detachment of single cell susing microarrayed transparent electrodes2011

    • Author(s)
      J. Fukuda, Y. Kameoka, H. Suzuki
    • Journal Title

      Biomaterials (IF=7.88)

      Volume: 32(28) Pages: 6663-9

    • DOI

      DOI:10.1016/j.biomaterials.2011.05.068

  • [Journal Article] Khademhosseini, SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures2011

    • Author(s)
      N. Sadr, M. Zhu, T. Osaki, T. Kakegawa, Y. Yang, M. Moretti, J. Fukuda, A.
    • Journal Title

      Biomaterials (IF=7.88)

      Volume: 32(30) Pages: 7479-90

    • DOI

      DOI:10.1016/j.biomaterials.2011.06.034

  • [Journal Article] Khademhosseini, Directed assembly of cell-laden microgels for building porous three-dimensional tissue constructs2011

    • Author(s)
      F. Yanagawa, H. Kaji, Y. H. Jang, H. Bae, D. Yanan, J. Fukuda, H. Qi, A.
    • Journal Title

      Khademhosseini, Directed assembly of cell-laden microgels for building porous three-dimensional tissue constructs

      Volume: 97A (1) Pages: 93-102

    • DOI

      DOI:10.1002/jbm.a.33034

  • [Journal Article] Hepatocytespheroid arrays inside microwells connected with microchannels2011

    • Author(s)
      J. Fukuda and K. Nakazawa
    • Journal Title

      Biomicrofluidics(IF=3.90)

      Volume: 5, 022205

    • DOI

      DOI:10.1063/1.3576905

  • [Journal Article] Directed assembly of cell-Laden hydrogels for engineeringfunctional tissues2010

    • Author(s)
      N. Kachouie, Y. Du, H. Bae, M. Khabiry, A. F. Ahari, B. Zamanian, J. Fukuda, and A. Khademhosseini
    • Journal Title

      Organogenesis

      Volume: 6(4) Pages: 234-44

    • DOI

      DOI:10.4161/org.6.4.12650

  • [Presentation] 両性イオンペプチド分子層の還元脱離を利用した細胞組織の構築2012

    • Author(s)
      福田淳二、掛川貴弘、望月直人、大崎達哉、鈴木博章
    • Organizer
      電気化学会第79回大会
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2012-03-29
  • [Presentation] Surface Engineering for Rapid Cell Detachment by Using Electrochemical Desorption of a Zwitterionic Oligopeptide Layer2011

    • Author(s)
      T. Kakegawa, H. Suzuki, J. Fukuda
    • Organizer
      MRS Fall Meeting & Exhibit 2011
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-12-01
  • [Presentation] 電気化学的な細胞脱離技術を用いたティッシュエンジニアリング2011

    • Author(s)
      望月直人、大崎達哉、掛川貴弘、鈴木博章、福田淳二
    • Organizer
      第49回日本人工臓器学会大会
    • Place of Presentation
      都市センターホテル
    • Year and Date
      2011-11-26
  • [Presentation] 電気化学的な細胞脱離を利用した細胞シート回収技術2011

    • Author(s)
      望月直人、鈴木博章、福田淳二
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ
    • Year and Date
      2011-11-02
  • [Presentation] 自己組織化単分子膜の電気化学還元脱離を用いた細胞脱離技術2011

    • Author(s)
      福田淳二、望月直人、掛川貴弘、鈴木博章
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ
    • Year and Date
      2011-11-02
  • [Presentation] Cell Detachment along with Electrical Cleavageof a Zwitterionic Oligopeptide Layer2011

    • Author(s)
      T. Kakegawa, H. Suzuki, J. Fukuda
    • Organizer
      Biofabrication 2011
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2011-10-08
  • [Presentation] Microfluidic system for mastitic diagnosis based on superoxide secretion activity of neutrophils2011

    • Author(s)
      S. Kimura, J. Fukuda, A. Tajima, H. Suzuki
    • Organizer
      Biofabrication 2011
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2011-10-08
  • [Presentation] 細胞遊走試験のためのトランスフェクションアレイチップ2011

    • Author(s)
      榎本詢子、藤田聡史、長崎玲子、鈴木博章、福田淳二
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-28
  • [Presentation] 微細加工技術を利用した細胞培養チップ2011

    • Author(s)
      福田淳二
    • Organizer
      第63回日本生物工学会大会(招待講演)
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-28
  • [Presentation] On-demand Detachment of Single Cells Using Microarrayed ITO Electrodes2011

    • Author(s)
      J Fukuda, Y Kameoka, H Suzuki
    • Organizer
      Asian Biomaterials Congress
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2011-09-17
  • [Presentation] 光架橋性ハイドロゲルを用いた血管網構築の試み2011

    • Author(s)
      大崎達哉、鈴木博章、福田淳二
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋工業大学
    • Year and Date
      2011-09-14
  • [Presentation] Electrical Detachment of cells forEngineering Capillary-Like Structures in a Photocrosslinkable Hydrogel2011

    • Author(s)
      T. Osaki, T. Kakegawa, H. Suzuki & J. Fukuda
    • Organizer
      International Conference of the IEEE Engineering in Medicine and Biology Society
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-09-01
  • [Presentation] Electrical Detachment of cells for Engineering Capillary-Like Structures in a Photocrosslinkable Hydrogel2011

    • Author(s)
      J Fukuda, Y Kameoka, H Suzuki
    • Organizer
      TERMIS-AP 2011
    • Place of Presentation
      Singapore, Singapore
    • Year and Date
      2011-08-04
  • [Presentation] Spatio-Temporal Cell Detachment from Culture Surface Using Microarrayed Transparent Electrodes2011

    • Author(s)
      T. Osaki, T. Kakegawa, N. Sadr, A.Khademhosseini, H. Suzuki & J. Fukuda
    • Organizer
      TERMIS-AP 2011
    • Place of Presentation
      Singapore, Singapore
    • Year and Date
      2011-08-04
  • [Presentation] 自己組織化単分子膜の還元脱離を利用した細胞組織体の構築2011

    • Author(s)
      福田淳二,掛川貴弘,望月直人,大崎達哉,鈴木博章
    • Organizer
      電気化学会第78回大会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2011-03-31
  • [Presentation] 電気化学細胞脱離を利用した血管様構築の作製技術2011

    • Author(s)
      福田淳二、大崎達哉、掛川貴弘、望月直人、鈴木博章
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2011-03-02
  • [Presentation] 電気化学細胞脱離を利用したティッシュエンジニアリング2011

    • Author(s)
      福田淳二
    • Organizer
      日本動物実験代替法学会第23回大会
    • Place of Presentation
      北里大学
    • Year and Date
      2011-03-02
  • [Presentation] Fabrication of Thick Cell Sheet via Electrochemical Reactions on Porous Membrane Culture Substrate2010

    • Author(s)
      N. Mochizuki, H. Suzuki, J. Fukuda
    • Organizer
      2010 MRS Fall meeting
    • Place of Presentation
      Boston, USA
    • Year and Date
      2010-11-29
  • [Presentation] 電気化学的原理に基づく細胞シート回収技術2010

    • Author(s)
      望月直人、鈴木博章、福田淳二
    • Organizer
      第62回日本生物工学会
    • Place of Presentation
      宮崎シーガイア
    • Year and Date
      2010-10-29
  • [Presentation] Fabrication of Thick Cell Sheet via Electrochemical Reactions on Porous Membrane Culture Substrate2010

    • Author(s)
      N. Mochizuki, H. Suzuki, J. Fukuda
    • Organizer
      TERMIS-AP 2010
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2010-09-17
  • [Presentation] Cell Detachment Along with Electrical Cleavage of a Zwitterionic Oligopeptide Layer2010

    • Author(s)
      T. Kakegawa, H. Suzuki, J. Fukuda
    • Organizer
      TERMIS-AP 2010
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2010-09-17
  • [Presentation] 自己組織化オリゴペプチドを用いた細胞接着制御2010

    • Author(s)
      掛川貴弘、鈴木博章、福田淳二
    • Organizer
      化学工学会第42回秋季大会
    • Place of Presentation
      同志社大学
    • Year and Date
      2010-09-08
  • [Presentation] ITO電極を用いた電気化学的原理に基づく細胞脱離2010

    • Author(s)
      亀岡典哲、鈴木博章、福田淳二
    • Organizer
      化学工学会第42回秋季大会
    • Place of Presentation
      同志社大学
    • Year and Date
      2010-09-08
  • [Remarks]

    • URL

      http://www.ims.tsukuba.ac.jp/~szk_fkd_lab/index.html

  • [Patent(Industrial Property Rights)] Cell culture method and device2011

    • Inventor(s)
      J Fukuda, H Suzuki, N Mochizuki, TKakegawa
    • Industrial Property Rights Holder
      University of Tsukuba
    • Industrial Property Number
      PCT出願、PCT/JP2011/070533
    • Filing Date
      2011-08-09
    • Overseas

URL: 

Published: 2013-07-31  

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