2011 Fiscal Year Final Research Report
Development of analysis method of methylation level using Zn finger protein
| Project/Area Number |
22656190
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HATA Kenichiro 国立成育医療研究センター研究所, 周産期病態研究部, 部長 (60360335)
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| Project Period (FY) |
2010 – 2011
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| Keywords | DNAのメチル化 / Znフィンガー蛋白質 / メチル化頻度解析 / 簡易迅速検出 / メチル化CpG / メチル化DNA結合蛋白質 |
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| Research Abstract |
We aimed to develop the method to analyze the DNA methylation level using Methylated DNA Binding protein(MBD) and Zinc finger protein fused luciferase(Zif-luciferase) and carried out the research as follows;
1) We developed the novel method for analyzing the DNA methylation level using MBD and Zif-luciferase.
2) We designed and constructed the novel Zif-luciferase which enable us the analysis of DNA methylation level of the gene region of androgen receptor which relates to the canceration.
3) We succeed in the analysis of the DNA methylation level Zif-luciferase. We also succeed in the analysis of the DNA methylation level of the p16 gene region which suppressing canceration. Therefore we can say our method is expected for broad application of the DNA methylation analysis of various target gene region.
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