Real-time monitoring system for gene expressions of the intracellular three genomes

2011 Fiscal Year Final Research Report

• Project/Area Number: 22657001
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Nagoya University
• Principal Investigator: ISHIURA Masahiro 名古屋大学, 遺伝子実験施設, 教授 (20132730)
• Co-Investigator(Renkei-kenkyūsha): MATSUO Takuya 名古屋大学, 遺伝子実験施設, 助教 (00452201)
• Project Period (FY): 2010 – 2011
• Keywords: ゲノム機能 / 発現

Research Abstract

Our study succeeded in developing a basic technology for simultaneous and real-time monitoring of the nuclear and chloroplast gene expressions in the green alga Chlamydomonas reinhardtii. This technology will be applied to studies on the photosynthesis and the creation of new bioenergy.

Research Products

• [Journal Article] Phase-dependent generation and transmission of time information by the KaiABC circadian clock oscillator through SasA-KaiC interaction in cyanobacteria (2012)
  • Author(s): J Valencia S, Bitou K, Ishii K, Murakami R, Morishita M, Onai K, Furukawa Y, Imada K, Namba K, Ishiura M.
  • Journal Title: Genes Cells Volume: 17 Pages: 398-419
  • Peer Reviewed
• [Journal Article] N-terminal acetyltransferase 3 gene is essential for robustcircadian rhythm of bioluminescence reporter in Chlamydomonas reinhardtii (2012)
  • Author(s): Matsuo T, Iida T, Ishiura M
  • Journal Title: Biochem Biophys Res Commun Volume: 418 Pages: 342-346
  • Peer Reviewed
• [Journal Article] Nonselective excitation of pulsed ELDOR using multi-frequency microwaves (2011)
  • Author(s): Asada Y, Mutoh R, Ishiura M, Mino H
  • Journal Title: J Magnetic Resonance Volume: 213 Pages: 200-205
  • Peer Reviewed
• [Journal Article] Thermodynamically induced conformational changes of the cyanobacterial circadian clock protein KaiB (2011)
  • Author(s): Mutoh R., Mino H., Murakami R., Uzumaki T., Ishiura M.
  • Journal Title: Applied Magnetic Resonance Volume: 40 Pages: 525-534
  • Peer Reviewed
• [Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophicgrowth of Synechocystis sp. PCC 6803 (2011)
  • Author(s): Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, Uozumi N
  • Journal Title: J Biol Chem Volume: 286 Pages: 25224-25235
  • Peer Reviewed
• [Journal Article] Chlamydomonas reinhardtii as a new model system for studying the molecular basis of the circadian clock (2011)
  • Author(s): Matsuo T, Ishiura M.
  • Journal Title: FEBS lett Volume: 585 Pages: 1495-1502
  • Peer Reviewed
• [Journal Article] 生物発光リアルタイム測定解析システム=有用生物株や有用遺伝子の網羅的な高速探索のための新システム= (2011)
  • Author(s): 小内清、石浦正寛
  • Journal Title: 光アライアンス「特集:生物フォトン研究の最前線」(3月号) Volume: 22 Pages: 20-26
• [Journal Article] 生物発光リアルタイム測定システムの歴史と展望～生物発光からゲノム学・生物物理学への潮流～ (2010)
  • Author(s): 小内清、石浦正寛
  • Journal Title: 生物物理 Volume: 50 Pages: 141-145
  • Peer Reviewed
• [Journal Article] New insights into the circadian clock in Chlamydomonas (2010)
  • Author(s): Matsuo T., Ishiura M.
  • Journal Title: Int. Rev. Cell Mol. Biol Volume: 280 Pages: 281-314
  • Peer Reviewed
• [Presentation] Identification and analysis of circadian clock genes in Chlamydomonas reinhardtii (2011)
  • Author(s): Matsuo T, Ishiura M
  • Organizer: Chlamydomonas and Phaeodactylum Workshop "Specific Light Driven Reactions in Unicellular Model Algae"
  • Place of Presentation: Jena, Germany
  • Year and Date: 20110325-27
• [Presentation] 緑藻の生物時計:起源、分子機構、時計研究モデルとしての可能性 (2011)
  • Author(s): 松尾拓哉、石浦正寛
  • Organizer: 第18回日本時間生物学会
  • Place of Presentation: 名古屋大学野依記念学術交流会館、名古屋
  • Year and Date: 2011-11-25
• [Presentation] 生物時計研究の新しいモデル生物:緑藻クラミドモナス、京都大学大学院薬学研究科、医薬創成情報科学講座 (2011)
  • Author(s): 松尾拓哉、石浦正寛
  • Organizer: システムバイオロジーセミナー
  • Place of Presentation: 京都大学薬学部、京都
  • Year and Date: 2011-10-03
• [Presentation] 藍色細菌と植物の生物時計の分子遺伝学的研究:時計分子装置のリアルタイム測定、構造、機能 (2011)
  • Author(s): 石浦正寛
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都大学、京都
  • Year and Date: 2011-09-21
• [Presentation] 藍色細菌Kai生物時計分子装置の時間発振中の構造変化 (2011)
  • Author(s): 村上怜子、石浦正寛
  • Organizer: 超異分野交流会
  • Place of Presentation: 東京、上智大学
  • Year and Date: 2011-03-19
• [Presentation] Masahiro Ishiura:Protein interactions in the in vitro cyanobacterial Kai clock system revealed by ESR analysis (2010)
  • Author(s): Risa Mutoh, Hiroyuki Mino, Reiko Murakami, Yuki Asada, Tatsuya Uzumaki, Kentaro Ishii, Masahiro Ishiura
  • Organizer: Asia-Pacific EPR/ESR Symposium
  • Place of Presentation: International Convention Center, Jeju, Republic of Korea, Poster
  • Year and Date: 20101010-14
• [Presentation] Analysis of the circadian clock genes ROCs in Chlamydomonas reinhardtii (2010)
  • Author(s): Matsuo T, Iida T, Kunii Y, Kato D, Tachikawa M, Ishiura M
  • Organizer: The 14th international Chlamydomonas conference
  • Place of Presentation: Wheaton College, Norton, Massachusetts, USA
  • Year and Date: 20100607-10
• [Presentation] 生物発光リアルタイム測定解析システムによる高等植物の油脂合成制御因子の網羅的探索 (2010)
  • Author(s): 河合都妙、小内清、石浦正寛、中村研三
  • Organizer: 科学技術交流財団「ハイスループットスクリーニングシステムと次世代DNAシーケンサーの相乗的応用」第1回研究会
  • Place of Presentation: 名古屋大学野依記念学術交流館、名古屋
  • Year and Date: 2010-07-23
• [Presentation] 生物発光リアルタイム測定解析システム-有用生物株や有用遺伝子の網羅的な高速探索のための新システムの開発- (2010)
  • Author(s): 小内清、石浦正寛
  • Organizer: 科学技術交流財団「ハイスループットスクリーニングシステムと次世代DNAシーケンサーの相乗的応用」第1回研究会
  • Place of Presentation: 名古屋大学野依記念学術交流館、名古屋
  • Year and Date: 2010-07-23
• [Presentation] 生物発光リアルタイム測定法の開発と生物時計研究への応用 (2010)
  • Author(s): 石浦正寛
  • Organizer: 岩手生物工学研究センター第165回公開セミナー
  • Place of Presentation: (財)岩手生物工学研究センター、北上市
  • Year and Date: 2010-05-28
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.jp/~ishiura-g/