2011 Fiscal Year Final Research Report
Analysis on cell filament formation and phenotypic plasticity of Escherichia coli using micro fluid system : rapid adaptation
| Project/Area Number |
22657006
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Ecology/Environment
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
SHIMADA Masakazu 東京大学, 大学院・総合文化研究科, 教授 (40178950)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Takehito 東京大学, 大学院・総合文化研究科, 准教授 (40447321)
WKAMOTO Yuichi 東京大学, 大学院・総合文化研究科, 准教授 (30517884)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | 1細胞培養計測系 / マイクロ流路 / 大腸菌 / 細胞伸長 / 表現型可塑性 / 捕食回避説 / 低密度良好説 / 環境ストレス説 |
|---|
| Research Abstract |
Using the on-chip one cell micro fluid system, we analyzed three potential factors affecting the cell filament formation of Escherichia coli ;(1) the enemy avoidance hypothesis,(2) the cell performance enhanced at a low density, and(3) the environmental stress. Either one factor or their combinations has/have been analyzed. First of all, we developed the device, so that too many growing cells can be automatically removed from the incubating area(Patent 2011-156767). Applying the device to the Tetrahymena-E. coli co-incubation system, we observed that the cell filament of E. coli forms when we used for two standard mediums for Tetrahymena(1/10PYG and CDM), even though we did not add Tetrahymena's pheromones at all.
|
