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2012 Fiscal Year Final Research Report

Identification of new determinants for double fertilization

Research Project

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Project/Area Number 22657017
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Morphology/Structure
Research InstitutionChiba University (2011-2012)
The Institute of Physical and Chemical Research (2010)

Principal Investigator

IGAWA Tomoko  千葉大学, 大学院・園芸学研究科, 助教 (00360488)

Research Collaborator YAMADA Lixy  名古屋大学, 大学院・理学研究科, 助教 (10551020)
MORI Toshiyuki  早稲田大学, 高等研究所, 助教 (00462739)
Project Period (FY) 2010 – 2012
Keywords重複受精 / 配偶子細胞 / ライブイメージング
Research Abstract

Arabidopsis marker lines, where gamete membrane was specifically visualized with fluorescent protein (GFP), were produced. Membrane proteins were purified from pistil tissue of the egg cell membrane- marker line by pull-down strategy using anti-GFP antibody. Among the identified proteins with putative trans-membrane region, one protein was found to be involved in seed fertility.

Research Products

(3 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Analysis of gamete membrane dynamics during double fertilization of Arabidopsis2013

    • Author(s)
      Tomoko Igawa, Yuki Yanagawa, Shin-ya Miyagishima and Toshiyuki Mori
    • Journal Title

      Journal of Plant Research

      Volume: 126巻 Pages: 387-394

    • DOI

      DOI:10.1007/s10265-012-0528-0

    • Peer Reviewed
  • [Journal Article] Gamete membrane dynamics during double fertilization in Arabidopsis2013

    • Author(s)
      Tomoko Igawa, Toshiyuki Mori
    • Journal Title

      Plant Signaling Behavior

      Volume: 8巻in press

    • URL

      http://www.landesbioscience.com/journals/psb/article/24512/

  • [Presentation] Live cell imaging analysis of gamete membrane behavior during double fertilization2012

    • Author(s)
      Tomoko Igawa, Toshiyuki Mori
    • Organizer
      International Symposium on the Mechanisms of sexual Reproduction in Animals and Plants
    • Place of Presentation
      Hotel Nagoya Garden Palace
    • Year and Date
      20121112-16

URL: 

Published: 2014-08-29   Modified: 2015-09-28  

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