2011 Fiscal Year Final Research Report
Analysis of photoreceptive ability in mouse brain based on functionally unknown rhodopsin-related proteins
| Project/Area Number |
22657022
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Osaka City University |
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Principal Investigator |
TERAKITA Akihisa 大阪市立大学, 大学院・理学研究科, 教授 (30212062)
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| Project Period (FY) |
2010 – 2011
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| Keywords | ロドプシ / 光受容 / シグナル伝達 / 視覚 / 非視覚 / 多様性 / 動物生理化学 |
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| Research Abstract |
It was reported that mRNA of an opsin, encephalopsin has been identified in brain of mammalian and non-mammalian vertebrates and therefore it is of interest to elucidate whether it actually serves as a light-sensor. In vitro experiments suggested that a member of encephalopsin group could act as a light-sensing G protein-coupled receptor. We successfully prepared transgenic mice that express green fluorescence protein(GFP) under regulation of upstream of encephalopsin gene and found GFP expression in the brain cells. The mice could facilitate to specifically isolate encephalopsin-expressing cells and characterize its photosensitivity.
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