2011 Fiscal Year Final Research Report
Nuclear genome analyses of Hatena arenicola using by next generation sequencing
| Project/Area Number |
22657024
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | University of Tsukuba |
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Principal Investigator |
INOUYE Isao 筑波大学, 生命環境系, 教授 (70168433)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Takeshi 筑波大学, 生命環境系, 講師 (40302369)
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| Project Period (FY) |
2010 – 2011
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| Keywords | ゲノム / 細胞内共生 / 進化 |
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| Research Abstract |
Hatena arenicola, a kathablepharid flagellate that maintains endosymbiotic algae temporary, is considered to be in the intermediate stage of secondary endosymbiosis. However, it is difficult to get an enough amount of DNA from H. arenicola because the flagellate cannot be cultured. Thus, we study the possibility of the genomic analyses using DNA amplified from single or a few cells. We succeeded to get genomic DNA sequences from plural cells of H. arenicola, but the most reads (about 90%) consisted of 2. 4 base pair repeated sequences. We performed BLASTx for the remaining 10% reads and identified possible sequences originated from Hatena genome. However, it was difficult to cover the whole nuclear genome of Hatena using these sequences, thus we concluded that further examination is necessary.
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[Presentation] NBRP「藻類」:日本における藻類の収集・保存・提供.ナショナルバイオリソースプロジェクトの活動2010
Author(s)
河地正伸, 川井浩史, 井上勲, 中山剛, 石田健一郎, 渡邉信, 羽生田岳昭, 山岸隆博, 甲斐厚, 中山卓郎, 笠井文絵
Organizer
第62回日本工学会大会
Place of Presentation
宮崎市シーガイヤ
Year and Date
20101027-29
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