2011 Fiscal Year Final Research Report
Study of Catalytic Mechanism of Hydrogen Activation by Hydrogenase using Neutron Crystal Structure Analysis
| Project/Area Number |
22657031
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
HIGUCHI Yoshiki 兵庫県立大学, 大学院・生命理学研究科, 教授 (90183574)
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| Project Period (FY) |
2010 – 2011
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| Keywords | タンパク質 / 中性子結晶解析 / 水素代謝 / 反応機構 |
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| Research Abstract |
The perfectly deuterated[NiFe] hydrogenase was tried to prepare by inoculating the sulfate-reducing bacterium in the deuterated media in 2010.However, it was not successful to obtain enough amount of the protein sample for crystallization trial. In 2011-2012, the ordinary(not deuterated) hydrogenase was tried to crystallize using deuterated precipitant in deuterated buffer solution. We have succeeded in preparation of a single crystal with a volume of 4 mm^3.This crystal gave a neutron diffraction pattern at 5.0 Aresolution at 100 K using the neutron beam at Japan Atomic Energy Research Institute. In addition, some parameters for diffraction experiment for large single crystals of[NiFe] hydrogenase at cryo-temperature have been found.
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Research Products
(64 results)
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[Journal Article] Three-dimensional Structure of Nylon Hydrolase and The Mechanism of Nylon-6 Hydrolysis2012
Author(s)
S. Negoro, N. Shibata., Y. Tanaka, K. Yasuhira, H. Shibata, H. Hashimoto, Y.-H. Lee, S. Oshima, R. Santa, S. Oshima, K. Mochiji, Y. Goto, T. Ikegami, K. Nagai, D. Kato, M. Takeo and Y. Higuchi
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 5079-5090
DOI
Peer Reviewed
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[Journal Article] Concerto Catalysis. Harmonising[NiFe] Hydrogenase and NiRu Model Catalysts2011
Author(s)
K. Ichikawa, K. Nonaka, T. Matsumoto, B. Kure, K-S. Yoon, Y. Higuchi, T. Yagi, S. OgoK. Ichikawa, K. Nonaka, T. Matsumoto, B. Kure, K-S. Yoon, Y. Higuchi, T. Yagi, S. Ogo
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Journal Title
Dalton Transactions
Volume: 39
Pages: 2993-2994
DOI
Peer Reviewed
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[Journal Article] Cytochrome c polymerization by successive domain swapping at the C-terminal helix2010
Author(s)
S. Hirota, Y. Hattori, S. Nagao, M. Taketa, H. Komori, H. Kamikubo, Z. Wang, I. Takahashi, S. Negi, Y. Sugiura, M. Kataoka, Y. Higuchi
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Journal Title
Pro. Natl. Acad. Sci
Volume: 107(29)
Pages: 12854-12859
DOI
Peer Reviewed
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[Journal Article] Crystal Structures of Ethanolamine Ammonia-lyase complexed with Coenzyme B_<12> Analogs and Substrates2010
Author(s)
N. Shibata, H. Tamagaki, N. Hieda, K. Akita, H. Komori, Y. Shomura, S. Terawaki, K. Mori, N. Yasuoka, Y. Higuchi, and T. Toraya
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Journal Title
J. Biol. Chem
Volume: 285(34)
Pages: 26484-26493
DOI
Peer Reviewed
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[Journal Article] X-ray Crystallographic Analysis of the 6-aminohexanoate Cyclic Dimer Hydrolase : Catalytic Mechanism and Evolution of an Enzyme Responsible for Nylon-6 Byproduct Degradation2010
Author(s)
K. Yasuhira, N. Shibata, G. Mongami, Y. Uedo, Y. Atsumi, Y. Kawashima, A. Hibino, Y. Tanaka, Y-H. Lee, D. Kato, M. Takeo, Y. Higuchi, and S. Negoro
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Journal Title
J. Biol. Chem
Volume: 285
Pages: 1239-1248
DOI
Peer Reviewed
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[Journal Article] Expression, Crystallization and Preliminary X-ray Crystallographic Study of Ethanolamine Ammonia-lyase from Escherichia coli2010
Author(s)
N. Shibata, H. Tamagaki, S. Ohtsuki, N. Hieda, K. Akita, H. Komori, Y. Shomura, S. Terawaki, T. Toraya, N. Yasuoka and Y. Higuchi
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Journal Title
Acta Crystallogr
Volume: F66
Pages: 709-711
DOI
Peer Reviewed
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