2012 Fiscal Year Final Research Report
Development of total genome exchange method in a bacterium.
| Project/Area Number |
22657040
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo (2011-2012)
Osaka University (2010) |
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Principal Investigator |
TABATA Kazuhito 東京大学, 大学院・工学系研究科, 助教 (50403001)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ゲノム / バクテリア / 再構成 |
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| Research Abstract |
We developed the method of exchanging a bacteria genome. In order to perform genome exchange, a host's genome must remove. So, we succeeded in preparation of the genome destructive digestion bacteria using a restriction enzyme expression system. Furthermore, we succeeded also in methylation of the genome to introduce and prepared the genome by which restriction enzyme is not cut. We tried to exchange of the genome using genome destruction bacteria and methylation genome. As a result, some colonies could be obtained. However, it was suggested as a result of the genome PCR or the sequence analysis of the genome that the genome of thesebacteria is a chimera of two genomes.
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[Presentation]2012
Author(s)
田端和仁、十河孝夫、野地博行
Organizer
生物物理学会第50回年会
Place of Presentation
名古屋・名古屋大学東山キャンパス
Year and Date
20120922-0924
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