2012 Fiscal Year Final Research Report
Development of a novel genome mapping method by using homologous recombination protein
| Project/Area Number |
22657044
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
MAESHIMA Kazuhiro 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (00392118)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATA Hideaki 大阪大学, 工学(系)研究科(研究院), 助教 (20455207)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 核酸 / ゲノム / 生体分子 |
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| Research Abstract |
To label a certain genomic region in the cell, a harsh condition to denature DNA is required. In this proposal, we tried to target a fluorescently tagged DNA sequence to the genomic DNA using some homologous recombination proteins. However, with homologous recombination protein RecA or XRad51, we did not get an efficient targeting in the fixed cells. As an alternative, we designed some fluorescent polyamides, which can recognize a certain DNA sequence, and succeeded to target efficiently the telomere repeats in the fixed cells
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Research Products
(8 results)
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[Journal Article] The organisation of genomic DNA in mitotic chromosomes: a novel view2013
Author(s)
Takata H., Matsunaga, S., Maeshima, K
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Journal Title
In Greilhuber, J., Leitch, I., Wendel, J.,Dolezel, J
Pages: 33-34
DOI
Peer Reviewed
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[Journal Article] Chromosomes without a 30-nm chromatin fiber2012
Author(s)
Joti, Y., Hikima, T., Nishino, Y., kamada, F., Hihara, S., Takata, H., Ishikawa, T., Maeshima, K.
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Journal Title
Nucleus
Volume: 3(5)
Pages: 404-410
DOI
Peer Reviewed
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