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2012 Fiscal Year Final Research Report

Metachronal wave of ciliary beats in a Paramecium cell.

Research Project

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Project/Area Number 22657049
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionYamaguchi University

Principal Investigator

IWADATE Yoshiaki  山口大学, 大学院・医学系研究科, 准教授 (40298170)

Project Period (FY) 2010 – 2012
Keywords繊毛 / 鞭毛 / メカノセンシング / ゾウリムシ / 繊毛波 / ケイジドカルシウム
Research Abstract

Ciliary movements in protozoa show metachronal coordination so as to maintain a constant phase difference between adjacent cilia. This coordination is called as “metachronal wave”.It is now generally thought that metachronal waves arise from hydrodynamic coupling between adjacent cilia at extracellular fluid. To confirm this, we planed to breakdown the hydrodynamic coupling of ciliary movements at a restricted portion of a Parameciumcell and observe whether metachronal coordination collapses or not. We applied UV irradiation to several cilia of a Parameciumcell, which was loaded caged calcium beforehand, to induce the reversal of beat-direction of the restricted cilia. Metachronal waves passed over the portion where the direction of ciliary movements was artificially reversed, suggesting that metachronal wave is mediated by something other than extracellular fluid.To clarify the other mediator, we sucked both ends of a Parameciumcell by two micropipettes and applied a vibration with a certain frequency to the one of the micropipettes. This induced cyclic stretching of cell body. The frequency of metachronal wave became equal to that of the cyclic stretching. These results suggest that not only extracellular fluid but also cell body can act as a coupling medium for propagation of metachronal waves.

  • Research Products

    (6 results)

All 2012 2011 Other

All Presentation (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] 自分自身の"力"による遊走細胞の前後極性形成2012

    • Author(s)
      岩楯好昭
    • Organizer
      第9回バイオオプティクス研究会(招待講演)
    • Place of Presentation
      山口大学(山口市)
    • Year and Date
      2012-12-07
  • [Presentation] 顕微鏡ステージ上で実行可能なエレクトロポレーション装置の開発2012

    • Author(s)
      継山晴進、岩楯好昭
    • Organizer
      第4回日本生物物理学会中国四国支部大会
    • Place of Presentation
      山口大学(山口市)
    • Year and Date
      2012-06-02
  • [Presentation] 人為的な細胞伸縮によるゾウリムシ繊毛のメタクロナールウェーブ周波数の制御2012

    • Author(s)
      馴松直紀、岩楯好昭
    • Organizer
      生体運動合同班会議
    • Place of Presentation
      筑波大学(つくば市)
    • Year and Date
      2012-01-06
  • [Presentation] メタクロナールウェーブは細胞膜の伸縮として伝播する2011

    • Author(s)
      馴松直紀、岩楯好昭
    • Organizer
      第44回原生動物学会大会
    • Place of Presentation
      奈良女子大学(奈良市)
    • Year and Date
      2011-11-11
  • [Remarks]

    • URL

      http://cellsystem.sci.yamaguchi-u.ac.jp/index.html

  • [Patent(Industrial Property Rights)] マイクロポレーション装置2012

    • Inventor(s)
      岩楯好昭、継山晴進
    • Industrial Property Rights Holder
      国立大学法人山口大学
    • Industrial Property Number
      特願 2012-077178
    • Filing Date
      2012-03-29

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Published: 2014-08-29  

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