2011 Fiscal Year Final Research Report
Construction of cell differentiation model by bacterial cells
| Project/Area Number |
22657059
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Evolutionary biology
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| Research Institution | Osaka University |
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Principal Investigator |
YING Beiwen 大阪大学, 大学院・情報科学研究科, 特任准教授(常勤) (90422401)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 発生 / 分化 / 進化 / 発現制御 / 大腸菌 / 遺伝子 / 細胞間相互作用 / 微生物 |
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| Research Abstract |
Cell differentiation is a process of stochasticity in phenotypic differentiation but shows the high homeostasis at the population level, i. e., the composition of varied differentiated cell types. A mutually inhibitory genetic circuit was designed and integrated into the E. coli genome. Also, the requirements for the amino acids(leucine and isoleucine) were introduced into the genetic circuit. The E. coli cells carrying this genetic circuit were used to mimic the cell differentiation successfully, based on the cell-to-cell communication.
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