Development of assay systems involving epigenetic control of gene expression to identify novel functional components in plants

2012 Fiscal Year Final Research Report

• Project/Area Number: 22658001
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Hokkaido University
• Principal Investigator: KANAZAWA Akira 北海道大学, 大学院・農学研究院, 准教授 (30281794)
• Project Period (FY): 2010 – 2012
• Keywords: 植物 / 機能性成分 / エピジェネティクス / シトシンメチル化 / 転写抑制

Research Abstract

In terms of finding novel functions of plant-derived compounds, assay systems for inhibitory activity of DNA methylation and transcriptional repression were developed using transgenic plants that carry an epigenetically silenced transgene. Through these assays, genistein, anisoflavonoid, was identified as the first plant-derived compound that had such an activity in plant cells.

Research Products

• [Journal Article] Deep sequencing uncovers commonality in small RNA profiles between transgene-induced and naturally occurring RNA silencing of chalcone synthase-A gene in petunia (2013)
  • Author(s): Kasai, M., Matsumura, H., Yoshida, K., Terauchi, R., Taneda, A. and Kanazawa, A
  • Journal Title: BMC Genomics Volume: 14 Pages: 63
  • DOI: doi:10.1186/1471-2164-14-63
  • Peer Reviewed
• [Journal Article] Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1orthologue using a virus vector in Nicotiana benthamiana (2013)
  • Author(s): Otagaki, S., Kasai, M., Masuta, C. and Kanazawa, A
  • Journal Title: Front. Genet Volume: 4 Pages: 44
  • DOI: doi:10.3389/fgene.2013.00044
  • Peer Reviewed
• [Journal Article] Induction of RNA-directed DNA methylation and heritable transcriptional gene silencing as a tool to engineer novel traits in plants (2013)
  • Author(s): Kasai, M. and Kanazawa, A
  • Journal Title: Plant Biotechnol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Complex genetic nature of sex-independent transmission ratio distortion in Asian rice species: the involvement of unlinked modifiers and sex-specific mechanisms (2012)
  • Author(s): Koide, Y., Shinya, Y., Ikenaga, M., Sawamura, N., Matsubara, K., Onishi, K., Kanazawa, A. and Sano, Y
  • Journal Title: Heredity Volume: 108 Pages: 242-247
  • DOI: doi:10.1038/hdy.2011.64
  • Peer Reviewed
• [Journal Article] Coincident sequence-specific RNA degradation of linked transgenes in the plant genome (2012)
  • Author(s): Kasai, M., Koseki, M., Goto, K., Masuta, C., Ishii, S., Hellens, R. P., Taneda, A. and Kanazawa, A
  • Journal Title: PlantMol. Biol Volume: 78 Pages: 259-273
  • DOI: doi:10.1007/s11103-011-9863-0
  • Peer Reviewed
• [Journal Article] In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation bygenistein (2012)
  • Author(s): Arase, S., Kasai, M. and Kanazawa, A
  • Journal Title: Plant Methods Volume: 8 Pages: 10
  • DOI: doi:10.1186/1746-4811-8-10
  • Peer Reviewed
• [Journal Article] An active CACTA-family transposable element is responsible for flower variegation in wild soybean Glycine soja (2012)
  • Author(s): Takahashi, R., Morita, Y., Nakayama, M., Kanazawa, A. and Abe, J
  • Journal Title: Plant Genome Volume: 5 Pages: 62-70
  • DOI: doi:10.3835/plantgenome2011.11.0028
  • Peer Reviewed
• [Journal Article] RNA silencing as a tool to uncover gene function and engineer novel traits in soybean (2012)
  • Author(s): Kasai, M. and Kanazawa, A
  • Journal Title: Breeding Sci Volume: 61 Pages: 468-479
  • DOI: doi:10.1270/jsbbs.61.468
  • Peer Reviewed
• [Journal Article] 植物改変の新技術,エピジェネティックな遺伝子発現制御 (2012)
  • Author(s): 金澤章、河西めぐみ
  • Journal Title: BIO INDUSTRY Volume: 29巻8号 Pages: 26-34
• [Journal Article] Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants (2011)
  • Author(s): Kanazawa, A., Inaba, J., Shimura, H., Otagaki, S., Tsukahara, S., Matsuzawa, A., Kim, B. M., Goto, K. and Masuta, C
  • Journal Title: Plant J Volume: 65 Pages: 156-168
  • DOI: doi:10.1111/j.1365-313X.2010.04401.x
  • Peer Reviewed
• [Journal Article] Establishment of an in vitro culture and particle bombardment-mediated transformation system in Miscanthus sinensis Anderss., a potential bioenergy crop (2011)
  • Author(s): Wang, X., Yamada, T., Kong, F., Abe, Y., Hoshino, Y., Sato, H., Takamizo, T., Kanazawa, A. and Yamada, T
  • Journal Title: Global Change Biology Bioenergy Volume: 3 Pages: 322-332
  • DOI: doi:10.1111/j.1757-1707.2011.01090.x
  • Peer Reviewed
• [Journal Article] Optimization of ion-beam irradiation for mutagenesis in soybean: effects on plant growth and production of visibly alteredmutants (2011)
  • Author(s): Arase, S., Hase, Y., Abe, J., Kasai, M., Yamada, T., Kitamura, K., Narumi, I., Tanaka, A. and Kanazawa, A
  • Journal Title: Plant Biotechnol Volume: 28 Pages: 323-329
  • DOI: doi:10.5511/plantbiotechnology.11.0111a
  • Peer Reviewed
• [Journal Article] RNA-mediated epigenetic modifications of an endogenous gene targeted by a viral vector: a potent gene silencing system to produce a plant that does not carry a transgene but has altered traits (2011)
  • Author(s): Kanazawa, A., Inaba, J., Kasai, M., Shimura, H. and Masuta, C
  • Journal Title: Plant Signal. Behav Volume: 6 Pages: 1090-1093
  • DOI: doi:10.4161/psb.6.8.16046
  • Peer Reviewed
• [Journal Article] Size and positional effects of promoter RNA segments on virus-induced RNA-directed DNA methylation and transcriptional genesilencing (2011)
  • Author(s): Otagaki, S., Kawai, M., Masuta, C. and Kanazawa, A
  • Journal Title: Epigenetics Volume: 6 Pages: 681-691
  • DOI: doi:10.4161/epi.6.6.16214
  • Peer Reviewed
• [Journal Article] 外来遺伝子を持たずに特定形質が変化した植物をつくる育種法 (2011)
  • Author(s): 金澤章
  • Journal Title: ニューカントリー Volume: 58巻693号 Pages: 70-71
• [Journal Article] Soybean stem growth habit gene Dt1 is an orthologue of Arabidopsis TFL1 (2010)
  • Author(s): Liu, B., Watanabe, S., Uchimiya, T., Kong, F., Kanazawa, A., Xia, Z., Nagamatsu, A., Arai, M., Yamada, T., Kitamura, K., Masuta, C., Harada, K. and Abe, J
  • Journal Title: Plant Physiol Volume: 153 Pages: 198-210
  • DOI: doi:10.1104/pp.109.150607
  • Peer Reviewed
• [Journal Article] Two coordinately regulated homologs of FLOWERING LOCUS T are involved in the control of photoperiodic flowering in soybean (2010)
  • Author(s): Kong, F., Liu, B., Xia, Z., Sato, S., Kim, B., Watanabe, S., Yamada, T., Tabata, S., Kanazawa, A., Harada, K. and Abe, J
  • Journal Title: Plant Physiol Volume: 154 Pages: 1220-1231
  • DOI: doi:10.1104/pp.110.160796
  • Peer Reviewed
• [Journal Article] Fertility restoration by Ifr1 in rice with BT-type cytoplasmic male sterility is associated with a reduced level, but not processing, of atp6-orf79 co-transcribed RNA (2010)
  • Author(s): Ohta, H., Ogino, A., Kasai, M., Sano, Y. andKanazawa, A
  • Journal Title: Plant Cell Rep Volume: 29 Pages: 359-369
  • DOI: doi:10.1007/s00299-010-0827-7
  • Peer Reviewed
• [Presentation] RNA-mediated epigenetic modifications by a viral vector:a potent system to produce a plant that does not carry a transgene but has altered traits (2012)
  • Author(s): Kanazawa, A., Inaba, J., Kasai, M., Shimura, H. and Masuta, C
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: ICC, Jeju, Republic of Korea
  • Year and Date: 2012-10-24
• [Presentation] Transcription and transposition of the soybean retrotransposon SORE-1 (2012)
  • Author(s): Tsuchiya, M., Yuan, H., Sato, M., Kasai, M., Abe, J. and Kanazawa, A
  • Organizer: 10th International Congress on Plant Molecular Biology.
  • Place of Presentation: ICC, Jeju, Republic of Korea
  • Year and Date: 2012-10-24
• [Presentation] Coincident RNA silencing of linked transgenes in the plant genome mediated by read-through transcription (2012)
  • Author(s): Kasai, M., Taneda, A. and Kanazawa, A
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: ICC, Jeju, Republic of Korea
  • Year and Date: 2012-10-23
• [Presentation] エピジェネティックな機構による転写抑制の解除を利用したinplantaアッセイ系により明らかになった植物成分のDNAメチル化抑制効果 (2012)
  • Author(s): 荒瀬幸子・河西めぐみ・金澤章
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡市九州大学
  • Year and Date: 2012-09-25
• [Presentation] ディープシークエンス解析によって明らかになった、ペチュニアにおけるコサプレッションとnaturallyoccurringRNAサイレンシングの低分子RNA産生機構の共通性 (2012)
  • Author(s): 河西めぐみ・松村英生・吉田健太郎・寺内良平・種田晃人・金澤章
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡市九州大学
  • Year and Date: 2012-09-25
• [Presentation] ペチュニア形質転換体における長期間の育成により誘導されたコサプレッションからの復帰 (2012)
  • Author(s): 河西めぐみ・金澤章
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都市京都産業大学
  • Year and Date: 2012-09-15
• [Presentation] エピジェネティックな遺伝子発現制御を介した形質改変 (2012)
  • Author(s): 金澤章・河西めぐみ
  • Organizer: 日本育種学会第54回シンポジウム「エピミュータジェネシスと次世代育種への展開」
  • Place of Presentation: 京都市京都産業大学
  • Year and Date: 2012-09-14
• [Presentation] RNA-mediated epigenetic modifications of an endogenous gene as a tool to produce a plant that does not carry a transgene but has altered traits (2011)
  • Author(s): Kanazawa, A., Inaba, J., Kasai, M., Shimura, H. and Masuta, C
  • Organizer: SOL&ICuGI2011 8th Solanaceae and 2nd CucurbitaceaeGenome Joint Conference
  • Place of Presentation: Kobe ConventionCenter, Kobe, Japan
  • Year and Date: 2011-12-01
• [Presentation] 外来遺伝子を持たずに特定の形質が変化した植物体の作出に必要なエピジェネティックな変化の促進 (2011)
  • Author(s): 金澤章・稲場純一・河西めぐみ・志村華子・増田税
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井市福井県立大学
  • Year and Date: 2011-09-24
• [Presentation] コサプレッションを誘導する遺伝子と共に導入した薬剤耐性遺伝子のRNAサイレンシング誘導機構 (2011)
  • Author(s): 河西めぐみ・種田晃人・金澤章
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井市福井県立大学
  • Year and Date: 2011-09-24
• [Presentation] 花の模様形成に現れるRNAサイレンシングの誘導過程 (2011)
  • Author(s): 金澤章・河西めぐみ
  • Organizer: 日本遺伝学会第83回大会・ワークショップ「植物の機能性RNA:花成から受精まで」
  • Place of Presentation: 京都府京都大学
  • Year and Date: 2011-09-22
• [Presentation] 低分子RNAの解析より示された隣接した2つのトランスジーンのRNAサイレンシングの誘導機構 (2011)
  • Author(s): 河西めぐみ・種田晃人・金澤章
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都府京都大学
  • Year and Date: 2011-09-20
• [Presentation] RNAサイレンシングによる遺伝子機能解析と形質改変 (2011)
  • Author(s): 金澤章
  • Organizer: 第29回日本植物細胞分子生物学会大会・シンポジウム「植物の逆遺伝学的解析技術はどこまで来たか」
  • Place of Presentation: 福岡市九州大学
  • Year and Date: 2011-09-07
• [Presentation] The inheritable transcriptional gene silencing targeted to an endogenous gene in petunia by using the Cucumber mosaic virus vector (2011)
  • Author(s): Inaba, J., Kanazawa, A., Shimura, H., Otagaki, S., Tsukahara, S., Matsuzawa, A., Kim, B., Goto, K. and Masuta, C
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Portola Hotel & Spa, Monterey, California, USA
  • Year and Date: 2011-03-22
• [Presentation] Read-through転写が関わる隣接したトランスジーン間の相互作用によるRNAサイレンシングの誘導 (2010)
  • Author(s): 河西めぐみ・種田晃人・金澤章
  • Organizer: 日本遺伝学会第82回大会
  • Place of Presentation: 札幌市北海道大学
  • Year and Date: 2010-09-20
• [Book] Gene duplication and RNA silencing in soybean. In A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships (2012)
  • Author(s): Kasai, M., Tsuchiya, M. and Kanazawa, A
  • Total Pages: 507-530
  • Publisher: Ed. by J. E. Board, InTech
• [Book] 植物の分子育種学 (2011)
  • Author(s): 金澤章
  • Total Pages: 123-134
  • Publisher: 講談社