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2012 Fiscal Year Final Research Report

New mutagenesis approaches to study gene function in crop

Research Project

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Project/Area Number 22658004
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionUniversity of Shizuoka

Principal Investigator

IIDA Shigeru  静岡県立大学, 大学院・食品栄養環境科学研究院, 客員教授 (30012777)

Co-Investigator(Renkei-kenkyūsha) KOBAYASHI Hirokazu  静岡県立大学, 大学院・食品栄養環境科学研究院, 教授 (80170348)
NOGUCHI Hiroshi  静岡県立大学, 薬学部, 教授 (60126141)
JOHZUKA Yasuyo (HISATOMI Yasuyo)  静岡県立大学, 大学院・食品栄養環境科学研究院, 客員研究員 (60517887)
Project Period (FY) 2010 – 2012
Keywords植物 / 突然変異 / 育種学 / 逆遺伝学 / バイオテクノロジー / 遺伝子
Research Abstract

To characterize gene function effectively, we tried to develop reverse genetic approaches, namely conditional knock-out and generation of subtle mutations by homologous recombination-mediated gene targeting and gene tagging employing endogenous nDart1-related DNA transposons. We also characterized insertion mutants isolated by gene targeting and tagging.

  • Research Products

    (13 results)

All 2013 2012 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results) Book (1 results)

  • [Journal Article] TAWAWA1, a regulator of rice inflorescence architecture, function through the suppression of meristem phase transition.2013

    • Author(s)
      A. Yoshida, M. Sasao, N. Yasuno, K. Takagi, Y. Daimon, R. Chen, R. Yamazaki, H. Tokunaga, Y. Kitaguchi, Y. Sato, Y. Nagamura, T. Ushijima, T. Kumamaru, S. Iida, M. Maekawa and J. Kyozuka
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 110 Pages: 767-772

    • DOI

      DOI:10.1073/pnas. 1216151110

    • Peer Reviewed
  • [Journal Article] A null mutation of ROS1a for DNA demethylation in rice is not transmittable to progeny.2012

    • Author(s)
      A. Ono, K. Yamaguchi, S. Fukada-Tanaka, R. Terada, T. Mitsui and S. Iida
    • Journal Title

      Plant J.

      Volume: 71 Pages: 564-574

    • DOI

      DOI:10.1111/j.1365-313X.2012.05009.x

    • Peer Reviewed
  • [Journal Article] Activation and epigenetic regulation of DNA transposon nDart1 in rice.2012

    • Author(s)
      C.-H. Eun, K. Takagi, K.-I. Park, M. Maekawa, S. Iidaand K. Tsugane
    • Journal Title

      Plant Cell Physiol.

      Volume: 53 Pages: 857-868

    • DOI

      DOI:10.1093/pcp/pcs060

    • Peer Reviewed
  • [Journal Article] Examination of transpositional activity of nDart1 at different stages of rice development.2011

    • Author(s)
      M. Hayashi-Tsugane, M. Maekawa, H. Kobayashi, S. Iidaand K. Tsugane
    • Journal Title

      Genes Genet, Syst.

      Volume: 86 Pages: 215-219

    • URL

      https://www.jstage.jst.go.jp/article/ggs/86/3/86_3_215/_article

    • Peer Reviewed
  • [Journal Article] Cre-loxP mediated marker elimination and gene reactivation at the waxy locus created in rice genome based on strong positive-negative selection.2010

    • Author(s)
      R. Terada, M. Nagahara, K. Furukawa, M. Shimamoto, K. Yamaguchi and S. Iida
    • Journal Title

      Plant Biotechnol.

      Volume: 27 Pages: 29-37

    • URL

      http://www.wdc-jp.biz/pdf_store/jspcmb/pdf/pb27_1/27_29.pdf

    • Peer Reviewed
  • [Presentation] イネ内在性DNAトランスポゾンnDart1のエピジェネティックな転移活性の制御2012

    • Author(s)
      栂根一夫、Eun Chang-Ho、高木恭子、栂根美佳、飯田滋
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都
    • Year and Date
      20120900
  • [Presentation] コシヒカリnDart1-0タグラインの育成2012

    • Author(s)
      西村秀希、氷見英子、飯田滋、栂根一夫、前川雅彦
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都
    • Year and Date
      20120900
  • [Presentation] DNAトランスポゾンnDart1挿入により生じたイネ半優性矮化多分げつ変異体の解析2011

    • Author(s)
      栂根(林)美佳、前川雅彦、飯田滋、栂根一夫
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111200
  • [Presentation] イオンビーム処理によるイネのDNAトランスポゾンDartの活性化2011

    • Author(s)
      前川雅彦、西村秀希、氷見英子、飯田滋、栂根一夫
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      福井
    • Year and Date
      20110900
  • [Presentation] アザシチジン処理で活性化した自律性因子Dartの特定2011

    • Author(s)
      西村秀希、氷見英子、飯田滋、栂根一夫、前川雅彦
    • Organizer
      第122回日本育種学会講演会
    • Place of Presentation
      福井
    • Year and Date
      20110900
  • [Presentation] Functional analysis of OsROS1a by homologous recombination-promoted gene targeting.2010

    • Author(s)
      A. Ono, K. Yamaguchi, Y. Johtsuka- Hisatomi, T. Mitsui, R. Terada and S. Iida
    • Organizer
      第33回日本分子生物学会年会及び第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101200
  • [Presentation] イネ内在性DNAトランスポゾンnDartの挿入部位指向性と遺伝子タギングへの応用2010

    • Author(s)
      栂根一夫、高木恭子、前川雅彦、飯田滋
    • Organizer
      イネ遺伝学・分子生物学ワークショップ2010
    • Place of Presentation
      つくば
    • Year and Date
      20100700
  • [Book] Effective contribution of the nDart transposon-tagging system to rice functional genomics. K. V. Urbano ed., Advances in Genetics Research 42011

    • Author(s)
      M. Maekawa, K. Tsugane and S. Iida
    • Total Pages
      259-272
    • Publisher
      Nova Science Publishers Inc., New York.

URL: 

Published: 2014-08-29  

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