2011 Fiscal Year Final Research Report
Genetic modification of Agrobacterium tumefaciens in order to use chloroplast transformation.
Project/Area Number |
22658025
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Applied microbiology
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Research Institution | Shinshu University |
Principal Investigator |
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Project Period (FY) |
2010 – 2011
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Keywords | 微生物遺伝・育種 / DNA伝達 |
Research Abstract |
Chloroplast transformation to incorporate a transgene into the chloroplast DNA and the transgene expression in chloroplast is suitable for high production of useful proteins in plants. Biolistic transformation with particle gun was used for chloroplast transformation. In this study, VIRD2 and VIRE2 proteins of Agrobacterium tumefaciens which transport bacterial T-DNA into plant nucleus were modified to convey the T-DNA into chloroplast. A. tumefaciens containing modified VIRD2 and VIRE2 would transform chloroplast of plants.
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