2011 Fiscal Year Final Research Report
Investigation of Chaperone Function of Phospholipids Containing Polyunsaturated Fatty Acids and Their Application to Overproduction of Membrane Proteins
| Project/Area Number |
22658028
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAMOTO Jun 京都大学, 化学研究所, 助教 (90511238)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 微生物 / 生体膜 / 高度不飽和脂肪酸 / エイコサペンタエン酸 / リン脂質 / 膜タンパク質 / フォールディング |
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| Research Abstract |
Shewanella livingstonensis Ac10 produces eicosapentaenoic acid-containing phospholipids (EPA-PLs) at low temperatures. We analyzed the role of EPA-PLs in the folding of Omp74, a major cold-inducible outer membrane protein of this bacterium. We found that the conformation of Omp74 in the mutant lacking EPA-PLs is different from that in the wild-type strain. In vitro experiments using liposomes showed that EPA-PLs function as a chemical chaperone in the membrane integration and folding of Omp74.
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Research Products
(18 results)
