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2011 Fiscal Year Final Research Report

Establishment of novel reaction mechanism on glycosylase and its application

Research Project

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Project/Area Number 22658032
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionHokkaido University

Principal Investigator

KIMURA Atsuo  北海道大学, 大学院・農学研究院, 教授 (90186312)

Project Period (FY) 2010 – 2011
Keywords反応機構 / 糖質分解酵素 / 反応中間体
Research Abstract

Two reaction mechanisms have been proposed : on glycosylases, i. e. oxo-carbenium ion-mediated mechanism and covalent linkage-formation mechanism. Recently, the latter one is supported by many glycosylase-scientists. However, there is a problem in the latter mechanism : this mechanism cannot be supported by organic chemistry-related knowledge. A purpose of this project is an experimental proof of the oxo-carbenium ion-mediated mechanism. Glycosylases catalyze the useful reaction of transglycosylation. This reaction mechanism may produce the novel application approach, the possibility of which is also investigated.

  • Research Products

    (28 results)

All 2012 2011 2010 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (10 results) Remarks (3 results)

  • [Journal Article] α-Glucosidase-catalyzed novel reaction on 1, 5-anhydrofructose, suggesting new metabolic pathway for production of glucose from starch2012

    • Author(s)
      Kim YM, Saburi W, Yu S, Nakai H, Maneesan J, Kang MS, Chiba S, Kim D, Okuyama M, Mori H, Kimura A
    • Journal Title

      J Biol Chem

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Structural elucidation of dextran degradation mechanism by Streptococcus mutans dextranase belonging to glycoside hydrolase family 662012

    • Author(s)
      Suzuki N, Kim YM, Fujimoto Z, Momma M, Okuyama M, Mori H, Funane K, Kimura A
    • Journal Title

      J Biol Chem

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Novel dextranase catalyzing cycloisomaltooligosaccharide-formation and its identification of catalytic amino acids and their functions using chemical rescue approach2012

    • Author(s)
      Kim YM, Kiso Y, Muraki T, Kang MS, Nakai H, Saburi W, Lang W, Kang HK, Okuyama M, Mori H, Suzuki R, Funane K, Suzuki N, Momma M, Fujimoto Z, Oguma T, Kobayashi M, Kim D, Kimura A
    • Journal Title

      J Biol Chem

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Glycoside hydrolase family 66 enzyme homolog from Bacteroides thetaiotaomicron VPI-5482 catalyzes endo-dextranolytic and cyclization activities2012

    • Author(s)
      Kim YM, Eiji Y, Kang MS, Nakai H, Saburi W, Okuyama M, Mori H, Funane K, Kim D, Kimura A
    • Journal Title

      FEBS J

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Biochemical characterization of a cycloisomaltooligosaccharide glucanotransferase from Paenibacillus sp. 598K2012

    • Author(s)
      Suzuki R, Terasawa K, Kimura K, Fujimoto Z, Momma M, Kobayashi M, Kimura A, Funane K
    • Journal Title

      Biochim Biophys Acta

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Novel quantitative method for the degree of branching in dextran2011

    • Author(s)
      Kim YM, Kimura A, Kim D
    • Journal Title

      Food Sci Biotechnol

      Volume: 20(2) Pages: 537-541

    • Peer Reviewed
  • [Journal Article] Bioengineered glucansucrase of Leuconostoc mesenteroides for preferred oligosaccharide synthesis using sucrose2011

    • Author(s)
      Kang HK, Kimura A, Kim D
    • Journal Title

      J Agric Food Chem

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Truncation of N-and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity2011

    • Author(s)
      Kim YM, Shimizu R, Nakai H, Mori H, Okuyama M, Kang MS, Fujimoto Z, Funane K, Kim D, Kimura A
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Calcium ion-dependent increase in thermostability of dextran glucosidase from Streptococcus mutans2011

    • Author(s)
      Kobayashi M, Hondoh H, Mori H, Saburi W, Okuyama M, Kimura A
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 75(8) Pages: 1557-1563

    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans2011

    • Author(s)
      Suzuki N, Kim YM, Fujimoto Z, Momma M, Kang HK, Funane K, Okuyama M, Mori H, Kimura A
    • Journal Title

      Acta Crystallogr F

      Volume: F67 Pages: 1542-1544

    • Peer Reviewed
  • [Journal Article] Function of C-terminal region of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulams T-30402011

    • Author(s)
      Funane K, Kawabata Y, Kim YM, Kang HK, Fujimoto Z, Kimura A, Kobayashi M
    • Journal Title

      Biochim Biophys Acta

      Volume: 1814 Pages: 428-434

    • Peer Reviewed
  • [Journal Article] 環状イソマルトオリゴ糖グルカノトランスフェラーゼの分子解析とBacillus circulans T-3040株における環状イソマルトオリゴ糖の新規な生合成経路の発見2011

    • Author(s)
      舟根和美, 川端康之, 鈴木龍一郎, 藤本瑞, 北岡本光, 木村淳夫, 小林幹彦
    • Journal Title

      応用糖質科学

      Volume: 1 Pages: 179-185

    • Peer Reviewed
  • [Journal Article] Suicide substrate-based inactivation of endodextranase byω-epoxyalkylα-D-glucopyranosides2010

    • Author(s)
      Kang HK, Kim YM, Nakai H, Kang MS, Hakamada W, Okuyama M, Mori H, Nishio T, Kimura A
    • Journal Title

      J Appl Glycosci

      Volume: 57 Pages: 111-117

    • Peer Reviewed
  • [Journal Article] Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities2010

    • Author(s)
      Ryu HJ, Jin X, Lee JH, Woo HJ, Kim YM, Kim GJ, Seo ES, Kang HK, Kim J, Cho DL, Kimura A, Kim D
    • Journal Title

      Enzyme Microb Technol

      Volume: 47 Pages: 212-215

    • Peer Reviewed
  • [Journal Article] Rice Os4BGlu12 is a wound-inducedβ-glucosidase that hydrolyzes cell-wall-β-glucan-derived oligosaccharides and glycosides2010

    • Author(s)
      Opassiri R, Maneesan J, Akiyama T, Pomthong B, Jin S, Kimura A, Ketudat-Cairns JR
    • Journal Title

      Plant Sci

      Volume: 179 Pages: 273-280

    • Peer Reviewed
  • [Presentation] 糖質酵素の分子機構に関する研究2012

    • Author(s)
      木村淳夫
    • Organizer
      日本応用糖質科学会平成24年度大会(第61回)
    • Place of Presentation
      東京農工大学(東京都)
    • Year and Date
      2012-09-20
  • [Presentation] Dietary influence of linearα-1, 6-polysaccharides on several parameters under experimental colitis depends on degree of the polymerization2011

    • Author(s)
      Hitoshi Iwaya, Jae-Sung Lee, Shinya Yamagishi, Aki Shinoki, Weeranuch Lang, Hee-Kwon Kang, Hiroshi Hara, Atsuo Kimura, Satoshi Ishizuka
    • Place of Presentation
      Royton Sapporo Hotel(Sapporo, Japan)
    • Year and Date
      20111114-17
  • [Presentation] Structural element determining the diverse specificities for chain-length of substrate in glycoside hydrolase family 31α-glucosidases2011

    • Author(s)
      Tagami T, Okuyama M, Mori H, Kimura A
    • Organizer
      9th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      Calouste Gulbenkian Foundation(Lisbon, Portugal)
    • Year and Date
      20110515-18
  • [Presentation] 環状イソマルトオリゴ糖グルカノトランスフェラーゼの合目的変異導入による生成物特異性の制御2011

    • Author(s)
      鈴木龍一郎,鈴木喜大,藤本瑞,門間充,木村啓太郎,木村淳夫,舟根和美
    • Organizer
      日本応用糖質科学会平成23年度大会(第60回)ポスター賞受賞
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2011-09-28
  • [Presentation] イソマルトメガロ糖とその生産酵素2011

    • Author(s)
      木村淳夫
    • Organizer
      メガロ糖・第2回シンポジウム「メガロ糖の製造と機能性ナノ材料:イソマルト型の新展開」
    • Place of Presentation
      帯畜大(帯広市)
    • Year and Date
      2011-09-16
  • [Presentation] Molecular mechanism of novel enzymes degrading and formingα-1, 6-glucosidic linkages, including megalosaccharide production2011

    • Author(s)
      Kim YM, Kanegae M, Hondoh H, Nishimura T, Saburi W, Sadahiro J, Lang W, Shimizu R, Nakai H, Okuyama M, Mori H, Kang MS, Fujimoto Z, Funane K, Kim D, A Kimura A
    • Organizer
      The 78th Annual Meeting and International Symposium in 2011 Annual Meeting of Korean Society of Food Science and Technology(KoSFoST)
    • Place of Presentation
      Daegu Exhibition Convention Center(Daegu Metropolitan City, Kora)
    • Year and Date
      2011-06-09
  • [Presentation] Dextran glucosidase and isomaltooligosaccharide 6-α-glucosyl transferase : mechanism of substrate recognition and transglucosylation2010

    • Author(s)
      Mori H, Hondoh H, Saburi W, Otsuka-Rachi H, Nishimura T, Kanegae M, YM Kim, Okuyama M, Kimura A
    • Organizer
      4th Symposium of theα-Amylase Family
    • Place of Presentation
      Smolenice Castle, Slovakia
    • Year and Date
      20100925-30
  • [Presentation] Putative role of Tyr227, Gln349 and Leu350 near catalytic amino acids of honeybeeα-glucosidase III2010

    • Author(s)
      Ngiwsara L, Iwai G, Mori H, Okuyama M, Kimura A
    • Organizer
      4th Symposium on the Alpha-Amylase Family
    • Place of Presentation
      Smolenice Cattle(Smolenice, Slovakia)
    • Year and Date
      20100925-30
  • [Presentation] 糖質酵素の基礎研究とその展開2010

    • Author(s)
      木村淳夫
    • Organizer
      日本応用糖質科学会平成22年度大会
    • Place of Presentation
      グランシップ(静岡県)
    • Year and Date
      2010-09-17
  • [Presentation] Molecular anatomy ofα-1, 6-glucoside(s)-forming and-hydrolyzing enzymes, comprising megalosaccharide-producing enzymes2010

    • Author(s)
      Young-Min Kim, Michiyo Kanegae, Hironori Hondoh, Takashi Nishimura, Wataru Saburi, Juri Sadahiro, Weeranuch Lang, Ryoko Shimizu, Hiroyuki Nakai, Hee-Kwon Kang, Masayuki Okuyama, Haruhide Mori, Min-Sun Kang, Zui Fujimoto, Kazumi Funane, Doman Kim, Kimura A
    • Organizer
      2011 CAB Agricultural Biotechnology Symposium on "Agricultural Biomaterials for Next Generation"
    • Place of Presentation
      Seoul Natinal University(Seoul, Korea)
    • Year and Date
      2010-08-25
  • [Remarks] アウトリーチ活動情報奥山正幸、木村淳夫:独立行政法人科学技術振興機構・平成23年度サイエンス・パートナーシップ・プロジェクト(SPP)事業(北海道札幌藻岩高校)アウトリーチ活動情報

  • [Remarks] 奥山正幸、木村淳夫:独立行政法人科学技術振興機構・平成22年度サイエンス・パートナーシップ・プロジェクト(SPP)事業(北海道札幌藻岩高校)アウトリーチ活動情報

  • [Remarks]

    • URL

      http://hecate.general.hokudai.ac.jp/welcome/toppage-jpn.html

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Published: 2013-07-31  

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