2011 Fiscal Year Final Research Report

Development of conductive quinone-modified Au-electrode enabling super-sensitive detection of the activities of mitochondrial respiratory enzymes

Research Project

Project/Area Number	22658039
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Bioproduction chemistry/Bioorganic chemistry
Research Institution	Kyoto University
Principal Investigator	MIYOSHI Hideto 京都大学, 大学院・農学研究科, 教授 (20190829)
Co-Investigator(Kenkyū-buntansha)	KANO Kenji 京都大学, 大学院・農学研究科, 教授 (10152828)
Project Period (FY)	2010 – 2011
Keywords	ミトコンドリア / 呼吸鎖酵素 / ユビキノン / 修飾電極
Research Abstract	Development of an assay system, which enables super-sensitive detection of the activities of respiratory enzymes in mitochondrial membrane, is very useful for the bioenergetic studies of highly limited amount of preparative mitochondria such as human tissues. To this end, we tried to make a conductive quinone-modified Au-electrode, which can detect the enzyme activities by monitoring a change in the redox-state of"quinone pool"of inner mitochondrial membrane.